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Recombinant Mouse IFN-gamma R2 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1185-GR

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1185-GR-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IFN-gamma R2 protein
Met1-Val243, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21

Predicted Molecular Mass

26 kDa (monomer)

SDS-PAGE

37-50 kDa, reducing conditions and visualized by silver stain.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse IFN-gamma R1 Fc Chimera (Catalog # 1026-GR) is immobilized at 2 μg/mL (100 μL/well), it binds to Recombinant Mouse IFN‑ gamma R2 in the presence of Recombinant Mouse IFN-gamma (Catalog # 485-MI). The concentration of Recombinant Mouse IFN‑ gamma R2 that produces 50% of the optimal binding response was found to be approximately 0.8-4 μg/mL.

Formulation, Preparation and Storage

1185-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R2

IFN‑ gamma R2 (Interferon gamma receptor 2; also called IFN‑ gamma R beta IFN‑ gamma RII, or AF1) is a 60‑64 kDa type I transmembrane glycoprotein that is a member of the class II cytokine receptor family of molecules (1). It is widely expressed as part of a preassembled cell surface multimeric complex. In the absence of IFN‑ gamma, the complex contains two each of IFN‑ gamma R1, R2 and Jak1 molecules (2). Binding of IFN‑ gamma to IFN‑ gamma R1 recruits Jak2 to IFN‑ gamma R2 and initiates phosphorylation, STAT1 binding, conformational changes, and transcriptional regulation, which mainly inhibits proliferation and/or promotes apoptosis (2, 3). Mouse IFN‑ gamma R2 cDNA encodes 332 amino acids (aa), including a signal sequence (aa 1‑27), an extracellular region (ECD, aa 28‑243) with two fibronectin type III domains, a transmembrane sequence (aa 244‑264) and a cytoplasmic tail (aa 265-332) (1, 2). Within the ECD, mouse IFN‑ gamma R2 shares 80% aa sequence identity with rat IFN‑ gamma R2, and 49‑55% with human, canine, porcine and bovine IFN‑ gamma R2. IFN‑ gamma R1 and R2 must be from the same species for receptor complexes to be active, and human IFN‑ gamma is not active on the mouse IFN‑ gamma receptor complex (1, 2). IFN‑ gamma R1 is essential for ligand binding and is more constitutively expressed, while IFN‑ gamma R2 is essential for signaling, and its more limited expression controls cell response to IFN‑ gamma (2, 3). For example, mouse T cell IFN‑ gamma R2 is down‑regulated during differentiation to subtypes such as Th1 which produce IFN‑ gamma. (3, 4) This allows expansion of activated cells without growth arrest due to paracrine response to IFN‑ gamma. Following expansion, IFN‑ gamma R2 is re‑expressed to limit the immune reaction (5). IFN‑ gamma signaling mediates control of intracellular pathogens such as mycobacteria (3, 4, 6). In humans, deficiency of IFN‑ gamma R2 or other IFN‑ gamma pathway molecules causes the MSMD (mendelian susceptibility to mycobacterial diseases) syndrome (6‑8).

References

  1. Hemmi, S. et al. (1994) Cell 76:803.
  2. Krause, C.D. et al. (2006) Cell Res. 16:55.
  3. Haring, J. S. et al. (2005) J. Immunol. 174:6791.
  4. Tau, G.Z. et al. (2000) J. Exp. Med. 192:977.
  5. Foulds, K.E. et al. (2008) J. Immunol. 180:842.
  6. Rosenzweig, S.D. et al. (2004) J. Immunol. 173:4000.
  7. Filipe-Santos. O. et al. (2006) Semin. Immunol. 18:347.
  8. Zhang, S-Y. et al. (2008) Immunol. Rev. 226:29.

Long Name

Interferon gamma Receptor 2

Alternate Names

AF-1, IFN-gR2, IFNgammaR2, IFNGR2, IFNGT1

Entrez Gene IDs

3460 (Human); 15980 (Mouse)

Gene Symbol

IFNGR2

UniProt

Additional IFN-gamma R2 Products

Product Documents for Recombinant Mouse IFN-gamma R2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IFN-gamma R2 Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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