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Recombinant Mouse IFN-gamma R1 Fc Chimera Protein, CF

Catalog # 1026-GR | R&D Systems, Inc. a Bio-Techne Brand
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1026-GR-100

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IFN-gamma R1/CD119 protein
Mouse IFN-gamma R1
(Ala26-Asp253)
Accession # Q91Y85
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala26

Predicted Molecular Mass

52.4 kDa (monomer)

SDS-PAGE

70-90 kDa, reducing conditions

Activity

Measured by its ability to inhibit IFN-gamma -mediated anti-viral activity using L‑929 mouse fibroblast cells. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
The ED50 for this effect is 6-36 ng/mL in the presence of 1 ng/mL of recombinant mouse IFN-gamma.

Formulation, Preparation and Storage

1026-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma  receptor complex is made up of two type I membrane proteins, IFN-gamma  R1 (IFN-gamma  R alpha) and IFN-gamma  R2 (IFN-gamma  R beta). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma  R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma  R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma  R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma  R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma  R1 is constitutively expressed in most cell types. Soluble IFN-gamma  R1 that binds IFN-gamma has been detected in biological fluids. The recombinant soluble IFN-gamma  R1 produced at R&D Systems has been shown to bind IFN-gamma with high affinity and is a potent IFN-gamma antagonist.

References

  1. Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.

Long Name

Interferon gamma Receptor 1

Alternate Names

CD119, IFN-gR1, IFNgammaR1, IFNGR1

Entrez Gene IDs

3459 (Human); 15979 (Mouse)

Gene Symbol

IFNGR1

UniProt

Product Documents for Recombinant Mouse IFN-gamma R1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IFN-gamma R1 Fc Chimera Protein, CF

For research use only

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