Recombinant Mouse IFN-gamma R1 Fc Chimera Protein, CF

Name: Recombinant Mouse IFN-gamma R1 Fc Chimera Protein, CF
Brand: rnd
SKU: 1026-GR

Catalog # 1026-GR

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	Q91Y85
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IFN-gamma R1/CD119 protein

Mouse IFN-gamma R1 (Ala26-Asp253) Accession # Q91Y85	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala26

Predicted Molecular Mass

52.4 kDa (monomer)

SDS-PAGE

70-90 kDa, reducing conditions

Activity

Measured by its ability to inhibit IFN-gamma -mediated anti-viral activity using L‑929 mouse fibroblast cells. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED₅₀ for this effect is 6-36 ng/mL in the presence of 1 ng/mL of recombinant mouse IFN-gamma.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1026-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids. The recombinant soluble IFN-gamma R1 produced at R&D Systems has been shown to bind IFN-gamma with high affinity and is a potent IFN-gamma antagonist.

Item	Value
References	Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.
Long Name	Interferon gamma Receptor 1
Entrez Gene IDs	3459 (Human); 15979 (Mouse)
Alternate Names	AVP, type 2, CD119, CD119 antigen, FLJ45734, IFN-gR1, IFN-gamma R1, IFN-gamma receptor 1, IFN-gamma-R1, IFNGR, IFNGR1, IFNgammaR1, immune interferon receptor 1, interferon gamma receptor 1, interferon-gamma receptor alpha chain, type 2

Citations (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IFN-gamma R1 Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Nachbur U et al. (2015), A RIPK2 inhibitor delays NOD signalling events yet prevents inflammatory cytokine production. Nat Commun, 6(0):6442.
PMID: 25778803
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Suzuki T et al. (2010), Importance of neonatal FcR in regulating the serum half-life of therapeutic proteins containing the Fc domain of human IgG1: a comparative study of the affinity of monoclonal antibodies and Fc-fusion proteins to human neonatal FcR. J. Immunol., 184(4):1968-76.
PMID: 20083659

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Recombinant Mouse IFN-gamma R1 Fc Chimera Protein, CF

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Product Specifications

Formulation, Preparation and Storage

Carrier Free

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Background: IFN-gamma R1/CD119

Citations (2)

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