Product Specifications for Recombinant Mouse IFN-alpha 9 Protein, CF
Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 9 protein Cys24-Glu190
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
18-24 kDa, under reducing conditions
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is < 100 pg/mL.
Scientific Data Examples for Recombinant Mouse IFN-alpha 9 Protein, CF
Recombinant Mouse IFN-alpha 9 Protein Bioactivity
Recombinant Mouse IFN-alpha 9 (10369-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is
Recombinant Mouse IFN-alpha 9 Protein SDS-PAGE
2 μg/lane of Recombinant Mouse IFN-alpha 9 (10369-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-24 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 100 μg/mL in PBS.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-alpha 9
Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). There are more than a dozen closely related IFN alpha subtypes found in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2, 3). The extracellular domain (ECD) of mature mouse IFNA9 consists of 166 amino acids (aa), with the closest homolog identified in humans as IFNA1. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (4, 5). Individual IFN alpha subtypes are known to display unique efficacies to viral protection. In gB DNA-Vaccinated mice, coimmunisation with IFNA9 has been shown to enhance protective immunity against cytomegalovirus and myocarditis (5).
Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
van Pesch, V. et al. (2004) J. Virol. 78:8219.
James C.M. et al. (2007) Vaccine. 25(10):1856.
Cull, V.S. et al. (2002) Gene Therapy. 9:1369-78.
Interferon alpha 9
Entrez Gene IDs
IFA9, IFN-alpha 9, IFNA9
Citations for Recombinant Mouse IFN-alpha 9 Protein, CF
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Product Documents for Recombinant Mouse IFN-alpha 9 Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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