Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Mouse IFN-alpha 4 (Mammalian) Protein

Bio-Techne includes R&D Systems | Catalog # 12115-1

A New rmIFN-alpha 4 is Available! It has lower endotoxin specifications, is made in-house, and is priced competitively!
Catalog #
Availability
Size / Price
Qty
Loading...
12115-1

Key Product Details

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source


Murine (C57/BI) DNA expressed in mammalian cells
Accession # NM_010504

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

19.5 kDa

Activity

Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Familletti et al. (1981) "A Convenience and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology , Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394] with the exception that EMCV rather than VSV was used as the challenge. The activity was determined relative to a lab standard of Mu-IFN-alpha A which was calibrated to the NIH Murine IFN-alpha standard (Ga02-901-511). Mouse (L929/EMCV) in this assay the EC50 for IFN is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay. Please note that IFN assays vary between labs and assay systems [Meager et al (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol Meth. 306:1]

Formulation, Preparation and Storage

12115-1
Formulation Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IFN-alpha 4

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name

Interferon alpha 4

Alternate Names

IFNA4, IFNalpha 4

Entrez Gene IDs

3441 (Human); 15967 (Mouse)

Gene Symbol

IFNA4

UniProt

Additional IFN-alpha 4 Products

Product Documents for Recombinant Mouse IFN-alpha 4 (Mammalian) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IFN-alpha 4 (Mammalian) Protein

For research use only

Citations for Recombinant Mouse IFN-alpha 4 (Mammalian) Protein (3)

Showing  1 - 3 of 3 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IFN-alpha 4 (Mammalian) Protein include:

There are no citations that match your criteria.

Showing  1 - 3 of 3 publications Showing All