Source	Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 4 protein Leu23-Glu186
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu23
Predicted Molecular Mass	19 kDa
SDS-PAGE	17-25 kDa, under reducing conditions
Activity	Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is <60 pg/mL.

Recombinant Mouse IFN-alpha 4 Protein, CF Scientific Data Examples

	Recombinant Mouse IFN-alpha 4 Protein Bioactivity Recombinant Mouse IFN-alpha/IFNA4 (Catalog # 10259-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <60 pg/mL.
	Recombinant Mouse IFN-alpha 4 Protein SDS-PAGE 2 μg/lane of Recombinant Mouse IFN-alpha 4 (Catalog # 10259-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17-25 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10259-IF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha 4

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors (1). The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFN-alpha subtypes and the various IFN-alphas share about 80% sequence homology among them (4, 5). The mouse IFN-alpha 4 consists of 186 amino acids (aa) including a 24 aa signal peptide and a 162 aa IFN-alpha 4 mature domain. The mature mouse IFN-alpha 4 shares 58% and 76% sequence identity with mature human and rat IFN-alpha 4, respectively. The mouse IFN-alpha 4 reduces the local replication of murine cytomegalovirus in the tibia muscle (6). mIFN-A4 is a strong activator of Mx gene and was shown to be very effective in reducing splenomegaly (7).

Item	Value
References	Pestka S, et al. (1987) Annu Rev Biochem. 56:727. Fung, K.Y. et al. (2013) Science 339:1088. Matsumiya, T. et al. (2007) J. Immunol. 179:4542. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139. van Pesch, V. et al. (2004) J. Virol. 78:8219. Yeow, W.S. et al. (1998) J. Immunol. 15:2932. Gerlach, N. et al. (2009) European J. Immunol. 39:136.
Long Name	Interferon alpha 4
Entrez Gene IDs	3441 (Human); 15967 (Mouse)
Alternate Names	IFN-alpha 4, IFN-alpha-4, IFN-alpha4a, IFNA4, IFNalpha 4, INFA4, Interferon alpha-4B, Interferon alpha-76, Interferon alpha-M1, MGC142200, interferon alpha-4, interferon, alpha 4

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Recombinant Mouse IFN-alpha 4 Protein, CF

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