Recombinant Mouse IFN-alpha 13 Protein, CF

Catalog # 10481-IF | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

Q80SU4.1

Source

HEK293

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 13 protein
Gly23-Glu189

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly23

Predicted Molecular Mass

19 kDa

SDS-PAGE

20-30 kDa, under reducing conditions

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is 1-15 ng/mL.

Scientific Data Images for Recombinant Mouse IFN-alpha 13 Protein, CF

Recombinant Mouse IFN-alpha 13 Protein Bioactivity

Recombinant Mouse IFN-alpha 13 Protein Bioactivity

Recombinant Mouse IFN-alpha 13 Protein (10481-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 1-15 ng/mL.
Recombinant Mouse IFN-alpha 13 Protein SDS-PAGE

Recombinant Mouse IFN-alpha 13 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IFN-alpha 13 (Catalog # 10481-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 20-30 kDa.

Formulation, Preparation and Storage

10481-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 13

Interferon-alpha 13 (IFN alpha-13), also known as IFN alpha-1/13 and LeIF D, is one of 14 subtypes within the IFN-alpha family (1). Interferons are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). The members of the IFN-alpha family are distinct but closely related proteins sharing approximately 80% amino acid (aa) sequence identity and a globular structure composed of five alpha-helices (1-3). The mature extracellular domain (ECD) of mouse IFN alpha-13 is shares 64% aa sequence identity with human IFN alpha-13. IFN-alpha family members signal through a common set of cell surface receptor complex composed of IFN alpha-R2 and IFN alpha-R1 subunits (2). Individual IFN-alpha subtypes are known to display unique efficacies to viral protection, and IFN alpha-13 displays antiviral activity protective against Theiler's virus, Mengo virus, VSV (4). IFN alpha-13 reproducibly appears to be up-regulated in response to TMEV infection (5). IFN alpha-13 also protects L929 cells against EMCV as tested in our assays.

References

  1. Moulton, V. R. (2016) Systemic Lupus Erythematosus, 137-141.
  2. Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.
  3. Pesch, V. et al. (2004). Journal of Virology, 78:8219.
  4. Pesch, V. et al. (2004). Journal of Biological Chemistry, 21:46321.
  5. Pesch, V. et al. (2001). Journal of Virology, 75:7811.

Long Name

Interferon alpha 13

Alternate Names

IFL, IFN-alpha 1b, IFNA13, IFNalpha 13

Entrez Gene IDs

3447 (Human); 230396 (Mouse); 298207 (Rat)

Gene Symbol

IFNA13

UniProt

Q80SU4.1

Product Documents for Recombinant Mouse IFN-alpha 13 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IFN-alpha 13 Protein, CF

For research use only

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