Recombinant Mouse Guanylyl Cyclase C/GUCY2C His Protein, CF

Name: Recombinant Mouse Guanylyl Cyclase C/GUCY2C His Protein, CF
Brand: R&D Systems
SKU: 11093-GC
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 11093-GC

Product Datasheet / COA / SDS

His-tag

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	Q3UWA6.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Guanylyl Cyclase C/GUCY2C His Protein, CF

Product Specifications for Recombinant Mouse Guanylyl Cyclase C/GUCY2C His Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived mouse Guanylyl Cyclase C/GUCY2C protein Val20-Gln430, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Val20
Predicted Molecular Mass	48 kDa
SDS-PAGE	85-100 kDa, under reducing conditions.
Activity	Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 2.5 μg/mL of protein is able to significantly inhibit neurite outgrowth.

Scientific Data Examples for Recombinant Mouse Guanylyl Cyclase C/GUCY2C His Protein, CF

	Recombinant Mouse Guanylyl Cyclase C/GUCY2C His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Mouse Guanylyl Cyclase C/GUCY2C His-tag Protein (Catalog # 11093-GC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-100 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11093-GC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 1.00 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Guanylyl Cyclase C/GUCY2C

Guanylyl Cyclase C (GUCY2C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (GC) family and helps regulate intestinal function (1, 8). There are 7 known members of the GC family (GC-A through GC-G) and they catalyze the conversion of guanosine triphosphate to cyclic guanosine monophosphate (cGMP) and pyrophosphate (1-4). Mature mouse GUCY2C consists of an extracellular domain (ECD) with a ligand binding domain, a transmembrane segment and a cytoplasmic region possessing a pseudokinase domain and a GC catalytic domain. The ECD of mature mouse GUCY2C shares 70% and 88% amino acid sequence identity with the ECD of human and rat GUCY2C, respectively. GUCY2C is primarily expressed in intestinal epithelial cells from the duodenum to rectum and binds to the endogenous gastrointestinal hormones guanylin and uroguanylin (1-4). Activation of GUCY2C signaling mediates fluid-ion homeostasis, intestinal inflammation, and cell proliferation in a cGMP-dependent manner (5). GUCY2C is also expressed in neurons, where it plays a role in attention deficiency/hyperactive behavior (6). GUCY2C is being investigated as a treatment for several gastrointestinal disorders including irritable bowel syndrome, obesity, colorectal cancer, and chronic idiopathic constipation (7, 8). Our Avi-tag Biotinylated GUCY2C features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Item	Value
References	Arshad, N. and Visweswariah S.S. (2012) FEBS Lett. 586:2835. Potter, L.R. (2011) Cell Signal. 2011 23:1921. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907. Gibbons, A.V. et al. (2013) Cancer Res. 73:22. Lucas, K. et al. (2000) Pharmacol Rev. 52:375. Gong, R. et al. (2011) Science. 333:1642. Waldman, S.A. and Camilleri, M. (2018) Gut. 67:1543. Kim, G.W. et al. (2013) Trends Endocrinol Metab. 24(4):165.
Entrez Gene IDs	2984 (Human); 14917 (Mouse); 25711 (Rat)
Alternate Names	DIAR6, EC 4.6.1, GC-C, GCC, GUC2C, GUC2CEC 4.6.1.2, GUCY2C, Guanylate Cyclase C, Guanylyl Cyclase C, Intestinal guanylate cyclase, MUCIL, STA Receptor, STARSTA receptor, StAR, guanylate cyclase 2C (heat stable enterotoxin receptor), hSTAR, heat-stable enterotoxin receptor

Citations for Recombinant Mouse Guanylyl Cyclase C/GUCY2C His Protein, CF

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Recombinant Mouse Guanylyl Cyclase C/GUCY2C His Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Product Specifications for Recombinant Mouse Guanylyl Cyclase C/GUCY2C His Protein, CF

Scientific Data Examples for Recombinant Mouse Guanylyl Cyclase C/GUCY2C His Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Guanylyl Cyclase C/GUCY2C

Citations for Recombinant Mouse Guanylyl Cyclase C/GUCY2C His Protein, CF

Product Documents for Recombinant Mouse Guanylyl Cyclase C/GUCY2C His Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected