Product Specifications for Recombinant Mouse Glypican 2 Protein, CF
Mouse myeloma cell line, NS0-derived mouse Glypican 2 protein Lys27-Arg358 & Ala359-Leu554, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Lys27 & Ala359
Predicted Molecular Mass
60 kDa, non-reducing conditions
Measured by its binding ability in a functional ELISA. Immobilized rmGlypican 2 at 4 µg/mL (100 µL/well) can bind rhMidkine with a linear range of 4-200 ng/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glypican 2
The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1 - GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35 - 63% sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54% sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17 - 25% sequence similarity (1 - 4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine-GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).
Filmus, J. and S.B. Selleck (2001) J. Clin. Invest. 108:497.
De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
Song, H. and J. Filmus (2002) Biochem. Biophys. Acta 1573:241.
Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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