Recombinant Mouse Glypican 2 Protein, CF

Name: Recombinant Mouse Glypican 2 Protein, CF
Brand: R&D Systems
SKU: 2355-GP
Price: 417 USD

Catalog # 2355-GP | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	Q8BKV1
Applications:	Binding Activity

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Product Specifications for Recombinant Mouse Glypican 2 Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse Glypican 2 protein Lys27-Arg358 & Ala359-Leu554, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Lys27 & Ala359
Predicted Molecular Mass	58.9 kDa
SDS-PAGE	60 kDa, non-reducing conditions
Activity	Measured by its binding ability in a functional ELISA. Immobilized rmGlypican 2 at 4 µg/mL (100 µL/well) can bind rhMidkine with a linear range of 4-200 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2355-GP

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glypican 2

The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1 - GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35 - 63% sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54% sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17 - 25% sequence similarity (1 - 4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine-GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).

Item	Value
References	Filmus, J. and S.B. Selleck (2001) J. Clin. Invest. 108:497. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117. Song, H. and J. Filmus (2002) Biochem. Biophys. Acta 1573:241. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968. Ivins, J.K. et al. (1997) Dev. Biol. 184:320. Kurosawa, N. et al. (2001) Glycoconj. J. 18:499.
Entrez Gene IDs	221914 (Human); 71951 (Mouse)
Alternate Names	2410016G05Rik, Cerebroglycan, GPC2, Glypican 2

Citations for Recombinant Mouse Glypican 2 Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Glypican 2 Protein, CF include:

Species: Mouse
Sample Types: Antibody
Applications: ELISA (Standard)

S Lugert et al. (2017-04-25), Glypican-2 levels in cerebrospinal fluid predict the status of adult hippocampal neurogenesis Sci Rep, 2017-07(0):46543.
PMID: 28440309
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Bunin A et al. (2015-07-28), Protein Tyrosine Phosphatase PTPRS Is an Inhibitory Receptor on Human and Murine Plasmacytoid Dendritic Cells. Immunity, 2015-043(2):277-88.
PMID: 26231120

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Product Documents for Recombinant Mouse Glypican 2 Protein, CF

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Recombinant Mouse Glypican 2 Protein, CF

Catalog # 2355-GP | R&D Systems, Inc. a Bio-Techne Brand

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