Skip to main content

Recombinant Mouse GFR alpha-3 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 2645-FR

Catalog #
Availability
Size / Price
Qty
Loading...
2645-FR-050

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse GFR alpha-3/GDNF R alpha-3 protein
Met Mouse GFR alpha-3
(Glu34-Arg379)
Accession # AAB70931
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

66.2 kDa (monomer)

SDS-PAGE

70-75 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Mouse Artemin (Catalog # 1085-AR) at 1 µg/mL can bind Recombinant Mouse GFR alpha‑3/GDNF R alpha‑3 Fc Chimera with an apparent Kd <1 nM.

Formulation, Preparation and Storage

2645-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-3/GDNF R alpha-3

Mouse glial cell line-derived neurotrophic factor (GDNF) family receptor alpha 3 (GFR alpha-3, GDNF R alpha-3) is an approximately 50 kDa plasma membrane glycoprotein that is one of four GDNF receptors, all of which are glycosylphosphoinositol (GPI)-anchored, contain three conserved cysteine repeats, and promote survival of neurons (1‑4). It is synthesized as a 397 aa precursor with a 28 aa N‑terminal signal sequence, a 343 aa mature segment, and a C-terminal GPI attachment signal sequence (3‑5). Mouse GFR alpha-3 shares 94% aa sequence identity with rat GFR alpha-3 and 76‑81% aa sequence identity with human, equine, bovine and canine GFR alpha-3. GFR alpha-3 is expressed in the central nervous system only during the earliest stages of neurogenesis, while later it is predominantly found in developing and adult nociceptive sensory neurons (2, 4‑8). It is expressed with the shared GDNF co‑receptor, the Ret receptor tyrosine kinase, in the trigeminal ganglion, pituitary gland, thymus, lung, and duodenum (2, 4). Its ligand, the GDNF family ligand artemin, is primarily expressed by immature Schwann cells in the peripheral nervous system, and vascular smooth muscle cells, directing axonal projection of sympathetic neurons (6, 7). Artemin first binds GFR alpha-3, which recruits Ret, forming a signaling complex that is a pentamer containing one artemin, two GFR alpha-3, and two Ret molecules (1, 6, 9). Signals from this complex are required for the development and survival of the superior cervical ganglion (SCG) neurons, and deletion of mouse GFR alpha-3 results in deficits in the SCG and inhibited development of intestinal Peyer’s patches (6, 7, 10, 11).

References

  1. Wang, X. et al. (2006) Structure 14:1083.
  2. Naveilhan, P. et al. (1998) Proc. Natl. Acad. Sci. USA 95:1295.
  3. Nomoto, S. et al. (1998) Biochem. Biophys. Res. Commun. 244:849.
  4. Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
  5. Worby, C. et al. (1998) J. Biol. Chem. 273:3502.
  6. Baloh, R. et al. (1998) Neuron 21:1291.
  7. Honma, Y. et al. (2002) Neuron 35:267.
  8. Orozco, O.E. et al.(2001) Eur. J. Neurosci. 13:2177.
  9. Schlee, S. et al. (2006) Nat. Chem. Biol. 2:636.
  10. Nishino, J et al. (1999) Neuron 23:725.
  11. Veiga-Fernandez, H. et al. (2007) Nature 446:547.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 3

Alternate Names

GDNF R alpha-3, GFR alpha3, GFRa-3, GFRA3

Entrez Gene IDs

2676 (Human); 14587 (Mouse)

Gene Symbol

GFRA3

UniProt

Additional GFR alpha-3/GDNF R alpha-3 Products

Product Documents for Recombinant Mouse GFR alpha-3 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse GFR alpha-3 Fc Chimera Protein, CF

For research use only

Loading...
Loading...
Loading...