Recombinant Mouse Artemin Protein

Name: Recombinant Mouse Artemin Protein
Brand: R&D Systems
SKU: 1085-AR

Catalog # 1085-AR

Citations (7)

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Key Product Details

Source:	E. coli
Accession #:	Q9Z0L2.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity, Bioactivity

View Full Technical Details for Recombinant Mouse Artemin Protein

Product Specifications

Source	E. coli-derived mouse Artemin protein Ala112-Gly224
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala112
Predicted Molecular Mass	12 kDa (monomer)
Activity	Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED₅₀ for this effect is 2-8 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse GFR alpha ‑3/GDNF R alpha ‑3 Fc Chimera (Catalog # 2645-FR) at 1 µg/mL (100 µL/well) can bind Recombinant Mouse Artemin with an apparent K_D <1 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1085-AR

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1085-AR/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Artemin

Artemin is a member of the Glia Cell-Derived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor beta (TGF-beta ) superfamily (1-4). Similar to other TGF-beta family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide-linked homodimer of the carboxy-terminal 113 amino acid residues. Mature mouse Artemin shares 88.5% amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40% amino acid sequence identity with the other three members of the GDNF family ligands (5). Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFR alpha -1-GFR alpha -4) and a common signaling component, cRET (receptor tyrosine kinase) (5-8). Artemin prefers to bind to GFR alpha -3 and activites the GFR alpha -3-RET. However, in the presence of RET, it can bind to GFR alpha -1 as well (4, 5, 9). Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs (5, 10, 11).

Item	Value
References	Lin, L-F.H. et al. (1993) Science 260:1130. Milbrandt, J. et al. (1998) Neuron 20:245. Kotzbauer, P.T. et al. (1996) Nature 384:467. Baloh, R.H. et al. (1998) Neuron 21:1291. Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361. Baloh, R.H. et al. (1997) Neuron 18:793. Jing, S. et al. (1996) Cell 85:1113. Jing, S. et al. (1997) J Biol Chem 272:33111. Nishino, J. et al. (1999) Neuron 23:725. Enomoto, H. et al. (2001) Development 128:3963. Andres, R. et al. (2001) Development 128:3685.
Entrez Gene IDs	9048 (Human); 11876 (Mouse)
Alternate Names	ARTN, Artemin, EVN, EVNneurotrophic factor, Enovin, NBN, Neublastin

Citations for Recombinant Mouse Artemin Protein (7)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Artemin Protein include:

Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Y Han et al. (2018), Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression Cell, 0(0).
PMID: 29606356
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Schaller et al. (2017), Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 114(12):E2486-E2493.
PMID: 28270618
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

EK Lippoldt et al. (2016), Inflammatory and neuropathic cold allodynia are selectively mediated by the neurotrophic factor receptor GFR?3 Proc Natl Acad Sci USA, 113(16):4506-11.
PMID: 27051069
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Ikeda-Miyagawa Y et al. (2015), Peripherally increased artemin is a key regulator of TRPA1/V1 expression in primary afferent neurons. Mol Pain, 11(0):8.
PMID: 25889103
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Bohorquez D et al. (2014), An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy. PLoS ONE, 9(2):e89881.
PMID: 24587096
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Li J et al. (2009), Autocrine regulation of early embryonic development by the artemin-GFRA3 (GDNF family receptor-alpha 3) signaling system in mice. FEBS Lett., 583(15):2479-85.
PMID: 19580811
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Malin SA et al. (2006), Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo. J. Neurosci., 26(33):8588-99.
PMID: 16914685

Recombinant Mouse Artemin Protein

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Formulation, Preparation and Storage

Carrier Free

1085-AR

Carrier

1085-AR/CF

Carrier Free

Reconstitution Calculator

Background: Artemin

Citations for Recombinant Mouse Artemin Protein (7)

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