Recombinant Mouse FGL2 HA-tag Protein, CF

Name: Recombinant Mouse FGL2 HA-tag Protein, CF
Brand: R&D Systems
SKU: 10691-FL

Catalog # 10691-FL

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	NS0
Accession #:	P12804.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse FGL2 HA-tag Protein, CF

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FGL2 protein

Hemagglutinin Tag (YPYDVPDYA)	Mouse FGL2 (Val20-Pro432) Accession # P12804.1
N-terminus	C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Tyr of HA-tag and Asp 31

Predicted Molecular Mass

48 kDa

SDS-PAGE

61-72 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Fc gamma RIIB/CD32b (Catalog # 1460-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse FGL2 HA-tag (Catalog # 10691-FL) binds with an ED₅₀ of 0.1-0.8 µg/mL. Measured by its ability to inhibit anti-CD3-induced proliferation of mouse T lymphocytes. The ED₅₀ for this effect is 1.5-12 µg/mL.

Recombinant Mouse FGL2 HA-tag Protein, CF Scientific Data Examples

	Recombinant Mouse FGL2 HA-tag Protein Binding Activity. When Recombinant Mouse Fc gamma RIIB/CD32b (1460-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse FGL2 HA-tag (Catalog # 10691-FL) binds with an ED₅₀ of 0.1‑0.8 µg/mL.
	Recombinant Mouse FGL2 HA-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Mouse FGL2 HA-tag (Catalog # 10691-FL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-72 kDa and 244-288 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10691-FL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: FGL2

FGL2 (fibrinogen-like protein 2), also called fibroleukin, is a 64-70 kDa secreted glycoprotein of the Fibrinogen-like superfamily. It has prothrombinase activity and also promotes T regulatory (Treg) activity (1-6). The mouse FGL2 gene encodes a 439 amino acid (aa) protein that contains a 23 aa signal sequence and a 416 aa mature sequence with an N-terminal coiled-coil region and a C-terminal fibrinogen homology domain or FRED (1, 2). A 260-280 kDa FGL2 complex is thought to be a tetramer formed by covalent disulfide linkage of dimers that are associated via coiled-coil interactions (2, 3). Mature mouse FGL2 shares 79% and 91% homology with human and rat FGL2 respectively. FGL2 appears to have two modes of action. One mode involves its prothrombinase activity, which requires calcium and acidic phospholipids (4). This mode is thought to be active during hepatitis viral infections when FGL2, produced by macrophages in response to IFN-gamma, induces hepatic apoptosis and fibrin deposition (7). In addition, FGL2 produced by endothelial cells in response to TNF-alpha within cardiac xenografts or allografts promotes coagulation during acute vascular rejection (7-9). A second mode of action involves soluble (not phospholipid-associated) FGL2 and is independent of prothrombinase activity (2). Soluble FGL2 is required for Treg function, and directly suppresses DC, T, and B cell immune reactivity; consequently, some FGL2-deficent mice develop autoimmune glomerulonephritis (5, 6). In vitro, soluble FGL2 can skew T cell polarization toward Th2 and inhibit proliferation of stimulated T cells and maturation of DC (6). In pregnancy, fetal trophoblast cells secrete FGL2. The immune suppressive mode of FGL2 may prevent early fetal loss; however, the procoagulant mode is thought to mediate infection-triggered abortion (10). In the central nervous system (CNS), FGL2 was shown to be highly expressed in glioma stem cells and primary glioblastoma cells and may serve as a critical immune oncology target (11).

Item	Value
References	Koyama, T. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1609. Marazzi, S. et al. (1998) J. Immunol.161:138. Olson, G. E. et al. (2004) J. Biol. Chem. 279:51266. Chan, C. W. Y. et al. (2002) J. Immunol. 168:5170. Shalev, I. et al. (2008) J. Immunol.180:249. Chan, C. W. Y. et al. (2003) J. Immunol. 170:4036. Liu, M. et al. (2006) J. Immunol. 176:7028. Mendicino, M. et al. (2005) Circulation 112:248. Ning, Q. et al. (2005) J. Immunol. 174:7403. Clark, D. A. et al. (2004) Mol. Hum. Reprod. 10:99. Yan, J. et al. (2019) Nat Commun. 10:448.
Long Name	Fibrinogen-like 2
Entrez Gene IDs	10875 (Human); 14190 (Mouse); 84586 (Rat); 102123631 (Cynomolgus Monkey)
Alternate Names	FGL2, Fibrinogen-like protein 2, Fibroleukin, T49, fibrinogen-like 2, pT49T49

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