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Recombinant Mouse Fc gamma RIIB/CD32b Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1460-CD

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1460-CD-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Fc gamma RIIB/CD32b protein
Thr30-Arg207, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr30

Predicted Molecular Mass

22 kDa

SDS-PAGE

30-40 kDa, reducing conditions

Activity

Measured by its ability to bind mouse IgG with an estimated KD 

Formulation, Preparation and Storage

1460-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIB/CD32b

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1 - 3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10-8 - 10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6 - 10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma RIIB, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three distinct genes encode the human CD32 group. These proteins share 94 - 99% amino acid identity in their extracellular domains but have divergent cytoplasmic domains and signaling capacities (1, 3). In contrast, a single gene encodes mouse CD32 (4). The protein product delivers an inhibitory signal upon ligand binding and is functionally equivalent to human Fc gamma RIIB (5). Mouse CD32 is the only Fc gamma R expressed on B cells and is also expressed on macrophages, neutrophils and mast cells. Mouse CD16 is closely related to mouse CD32 throughout its extracellular domain (95% amino acid identity), but has a divergent cytoplasmic domain and functions as an activating receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

References

  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.

Long Name

Fc gamma Receptor II

Alternate Names

CD32b, FCGR2B, FcgRIIB, FCRIIB

Entrez Gene IDs

2213 (Human); 14130 (Mouse); 289211 (Rat); 102139530 (Cynomolgus Monkey)

Gene Symbol

FCGR2B

UniProt

Additional Fc gamma RIIB/CD32b Products

Product Documents for Recombinant Mouse Fc gamma RIIB/CD32b Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Fc gamma RIIB/CD32b Protein, CF

For research use only

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