Source	Mouse myeloma cell line, NS0-derived mouse Fc alpha/mu R protein Trp47-Arg455, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Trp47
Predicted Molecular Mass	44 kDa
SDS-PAGE	97-112 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Mouse Fc alpha /μ R is immobilized at 2 μg/mL, 100 μL/well, the concentration of Mouse IgM that produces 50% of the optimal binding response is 0.04-0.24 μg/mL.

Recombinant Mouse Fc alpha/mu R His-tag Protein, CF Scientific Data Examples

	Recombinant Mouse Fc alpha/mu R His-tag Protein Binding Activity When Recombinant Mouse Fc alpha/mu R (Catalog # 10069-FC) is coated at 2ug/mL, 100 uL/well, Mouse IgM binds with an ED₅₀ of 0.04-0.24 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10069-FC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc alpha/mu R

Fc alpha/mu Receptor (FCAMR), designated CD351, is an approximately 60-kDa transmembrane protein that serves as a receptor for IgA and IgM immunoglobulins (1). Mature mouse FCAMR consists of a 420 amino acid (aa) extracellular domain with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 59 aa cytoplasmic domain. Within the ECD, mouse FCAMR shares 51% aa and 80% aa sequence identity with human and rat FCAMR, respectively. FCAMR is expressed on B cells, macrophages, and kidney mesangial cells (2-4). It binds to both IgA and IgM in immune complexes but not to monomeric immunoglobulin (2, 5). FCAMR participates in pathogen clearance as well as foam cell formation by mediating the internalization of IgM-opsonized microbes and oxidized LDL-containing particles (2, 6).

Item	Value
References	Wang, H. et al. (2016) Front. Immunol. 7:99. Shibuya, A. et al. (2000) Nat. Immunol. 1:441. Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104. McDonald, K.J. et al. (2002) Biochem. Biophys. Res. Commun. 290:438. Ghumra, A. et al. (2009) Eur. J. Immunol. 39:1147. Feng, X. et al. (2010) Atherosclerosis 208:396.
Long Name	Fc alpha/mu Receptor
Entrez Gene IDs	83953 (Human); 64435 (Mouse); 681070 (Rat)
Alternate Names	CD351, CD351 antigen, FCA/MR, FCAMR, FKSG87, Fc alpha/mu R, Fc alpha/mu receptor, Fc receptor, IgA, IgM, high affinity, high affinity immunoglobulin alpha and immunoglobulin mu Fc receptor, immunity related factor, receptor for Fc fragment of IgA and IgM, uR

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Recombinant Mouse Fc alpha/mu R His-tag Protein, CF

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