Recombinant Mouse DLL4 Fc Chimera Protein, CF

Name: Recombinant Mouse DLL4 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10089-D4

Catalog # 10089-D4

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	NS0
Accession #:	Q9JI71
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse DLL4 Fc Chimera Protein, CF

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse DLL4 protein

Mouse DLL4 (Gly27-Pro525) Accession # Q9JI71	IEGRMDP	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly27

Predicted Molecular Mass

81 kDa

SDS-PAGE

97-108 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED₅₀ for this effect is 0.02-0.2 μg/mL.

Recombinant Mouse DLL4 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Mouse DLL4 Fc Chimera Protein Bioactivity Immobilized Recombinant Mouse DLL4 Fc Chimera enhances Recombinant Human BMP-2 (Catalog # 355-BM) induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.02-0.2 µg/mL.
	Recombinant Mouse DLL4 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Mouse DLL4 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 97-108 kDa and 190-210 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10089-D4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

Item	Value
References	Shutter, J.R. et al. (2000) Genes Dev. 14:1313. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543. Walker, L. et al. (2001) Stem Cells 19:543. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.
Long Name	Delta-like 4
Entrez Gene IDs	54567 (Human); 54485 (Mouse); 100152163 (Porcine)
Alternate Names	DLL4, Delta 4 precursor, Drosophila Delta homolog 4, MGC126344, delta 4, delta ligand 4, delta-like 4 (Drosophila), delta-like 4 homolog, delta-like 4 homolog (Drosophila), delta-like 4 protein, delta-like protein 4, delta4, hdelta2, notch ligand DLL4, notch ligand delta-2

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