Product Specifications for Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein, CF
Mouse myeloma cell line, NS0-derived mouse CTRP9/C1qTNF9 protein
Mouse C1qTNF9 (Gln20-Ser333) Accession # Q4ZJN1
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
No results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Asp21.
Predicted Molecular Mass
39-43 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CTRP9/C1qTNF9 is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CL-K1/COLEC11 that produces 50% of the optimal binding response is 15-90 ng/mL.
Scientific Data Examples for Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein, CF
Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein Binding Activity
When Recombinant Mouse CTRP9/C1qTNF9 (Catalog # 2132-TN) is coated at 0.5 μg/mL, 100 μL/well, Biotinylated Recombinant Human CL-K1/COLEC11 binds with an ED50 of 15-90 ng/mL.
Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse CTRP9/C1qTNF9 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39-43 kDa and 39-43 & 80-90 kDa, respectively.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with Trehalose.
Reconstitute at 250 μg/mL in water.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
C1qTNF9, also known as CTRP9, is an approximately 40 kDa member of the C1q and TNF-related protein family (1). Like all members of this protein family, C1qTNF9 consists of a short variable region, a collagenous domain that can be hydroxylated, and a C1q-like globular domain (1). Mouse C1qTNF9 shares 86% and 98% amino acid sequence identity with the human and rat orthologs, respectively. Both the mouse and human C1qTNF9 proteins are expressed mostly in adipose tissue, but the mouse protein has also been detected in the heart, lung, muscle, kidney, testis, lymph node, smooth muscle, prostate, thymus, and uterus (1, 2). C1qTNF9 has been shown to be secreted as trimers and higher order multimers and also to form hetero-oligomers with Adiponectin (1, 2). Mouse C1qTNF9 can stimulate the phosphorylation of AMPK, Akt, and eNOS (1, 3, 4). Also in mice, C1qTNF9 may have an important role in cardiac and metabolic health. Its expression has a cardioprotective effect following acute myocardial infarction that may be dependent on AMPK activation (4-6). Additionally, transgenic mice overexpressing C1qTNF9 are resistant to high fat diet‑induced obesity (7). This metabolic role may be conserved, since C1qTNF9 serum levels have been shown to inversely correlate with metabolic syndrome in humans (8).
Wong, G.W. et al. (2009) FASEB J. 23:241.
Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.
Zheng, Q. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:2616.
Kambara, T. et al. (2012) J. Biol. Chem. 287:18965.
Su, H. et al. (2013) Basic Res. Cardiol. 108:315.
Sun, Y. et al. (2013) Circulation 128:S113.
Peterson, J.M. et al. (2013) Am. J. Physiol. Regul. Integr. Comp. Physiol. 305: R522.
Hwang, Y.C. et al. (2014) Int. J. Obes. (Lond) 38:1207
C1q and Tumor Necrosis Factor Related Protein 9
Entrez Gene IDs
338872 (Human); 239126 (Mouse)
AQL1, C1QTNF9AComplement C1q tumor necrosis factor-related protein 9, C1q and tumor necrosis factor related protein 9, C1qTNF9, CTRP9, MGC48915, complement C1q tumor necrosis factor-related protein 9A
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Product Documents for Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein, CF
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