Product Specifications for Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF
Mouse myeloma cell line, NS0-derived mouse CTRP4/C1qTNF4 protein Leu17-Leu326, with a C-terminal 6-His tag
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
34-38 kDa, reducing conditions
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse CTRP4/C1qTNF4, immobilized at 2.5-5 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
Scientific Data Examples for Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF
Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse CTRP4/C1qTNF4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-38 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with Trehalose.
Reconstitute at 500 μg/mL in water.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
3 months, ≤ -20 °C under sterile conditions after reconstitution.
C1qTNF4, also known as CTRP4, is an approximately 34 kDa member of the C1q family of secreted proteins (1, 2). C1qTNF4 consists of a signal peptide followed by two tandem globular C1q domains (1). It is the only C1q family member with tandem C1q domains (1, 2). The second C1q domain contains an RGD peptide, suggesting that it could potentially interact with integrins (3). Mature mouse C1qTNF4 shares 95% and 98% amino acid sequence identity with human and rat C1qTNF4, respectively. Expression of human C1qTNF4 occurs most abundantly in brain and adipose tissue (1). Neurons in the hypothalamus express and secrete CTRP4 which modulates food intake and energy balance by controlling neuropeptide gene expression (1). In-house finding shows CTRP4 can support neurite outgrowth of cortical neurons. Like other CTRP family members, C1qTNF4 can form dimers, trimers, hexamers, and high molecular weight oligomers following secretion (1, 4). Injection of recombinant C1qTNF4 into mice can reduce food intake, body weight, and ambulatory activity levels (1). C1qTNF4 has also been shown to promote human cancer cell survival in vitro (5).
Byerly, M.S. et al. (2014) J. Biol. Chem. 289:4055.
Kishore, U. et al. (2004) Trends Immunol. 25:551.
Ruoslahti, E. (1996) Annu. Rev. Cell Dev. Biol. 12:697.
Wong, G.W. et al. (2008) Biochem. J. 416:161.
Li, Q. et al. (2011) Cancer Lett. 308:203.
C1q And Tumor Necrosis Factor Related Protein 4
Entrez Gene IDs
114900 (Human); 67445 (Mouse); 311184 (Rat)
C1q and tumor necrosis factor related protein 4, C1qTNF4, CTRP4, CTRP4ZACRP4, ZACRP4, complement C1q tumor necrosis factor-related protein 4, complement-c1q tumor necrosis factor-related protein 4
Citations for Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF
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Product Documents for Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF
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