Can Recombinant Mouse CTLA-4 Fc Chimera Protein (Catalog # 434-CT) be used with human cell lines or any other species?

Name: Recombinant Mouse CTLA-4 Fc Chimera Protein
Brand: R&D Systems
SKU: 434-CT
Rating: 5 (1 reviews)

The activity of Recombinant Mouse CTLA-4 Fc Chimera Protein (Catalog # 434-CT) is measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.4 µg/mL when stimulated with 1 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) in the presence of PHA. The product shows activity on the human Jurkat cell line. For human assay systems, please also consider Recombinant Human CTLA-4 Fc Chimera His-tag Protein (Catalog # 325-CT) or Recombinant Human CTLA-4 Fc Chimera (Catalog #7268-CT). Please refer the Citations tab to find publications reporting the use of the protein on other species. Filter the results based on species and sample type for a more relevant search to your target species.

Recombinant Mouse CTLA-4 Fc Chimera Protein

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 434-CT

Citations (12)

(1)

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q6GTR6
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse CTLA-4 Fc Chimera Protein

Product Specifications for Recombinant Mouse CTLA-4 Fc Chimera Protein

Source

Mouse myeloma cell line, NS0-derived mouse CTLA-4 protein

Mouse CTLA-4 (Ala37-Phe162) Accession #Q6GTR6	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala37

Predicted Molecular Mass

41 kDa (monomer)

SDS-PAGE

55 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. Linsley, P.S. et al. (1991) J. Exp. Med. 174:561. The ED₅₀ for this effect is 0.1-0.4 µg/mL when stimulated with 1 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) in the presence of PHA.

Scientific Data Examples for Recombinant Mouse CTLA-4 Fc Chimera Protein

	Recombinant Mouse CTLA-4 Fc Chimera Protein Bioactivity Recombinant Mouse CTLA-4 Fc Chimera inhibits IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 0.03-0.15 µg/mL when stimulated with 1 µg/mL Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

434-CT

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

434-CT/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CTLA-4

CTLA-4 (cytotoxic T-lymphocyte-4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily (1, 2). Human or mouse CTLA-4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence. It is found as a covalent homodimer of 41 ‑ 43 kDa (2) Within the ECD, mouse CTLA-4 shares 94% and 68 ‑ 71% aa sequence identity with rat and human/porcine/bovine/rabbit/feline/canine CTLA-4, respectively. A 174 aa form that lacks TM and cytoplasmic sequences (sCTLA-4) is possibly secreted (3 ‑ 5). Isoforms of 56 ‑ 79 aa that mainly contain parts of the cytoplasmic domain are reported. In mouse, an isoform lacking the Ig-like domain has ligand-independent inhibitory activity and is termed liCTLA-4 (6). CD28, which is structurally related to CTLA-4, is constitutively expressed on naïve T cells and promotes T cell activation when engaged by B7-2 on antigen-presenting cells (APC) within the immunological synapse (IS) (1, 7, 8). In contrast, CTLA-4 is recruited from intracellular vesicles to the IS beginning 1-2 days after T cell activation (2, 7, 8). It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending T cell activation (9). Abatacept, a therapeutic human CTLA-4-Ig fusion protein (trade name Orencia), competes with CD28 for B7-1 and B7-2 binding and has been used to antagonize T cell activation in autoimmune conditions and to enhance transplant survival (10). Mice deleted for CTLA-4 show no abnormalities until after birth, but then develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells, which constitutively express CTLA-4 in wild-type mice and humans (11 ‑ 13).

Item	Value
References	Harper, K. et al. (1991) J. Immunol. 147:1037. Teft, W.A. et al. (2006) Annu. Rev. Immunol. 24:65. Magistrelli, G. et al. (1999) Eur. J. Immunol. 29:3596. Tector, M. et al. (2009) BMC Immunol. 10:51. Oaks, M.K. and K.M. Hallett (2000) J. Immunol. 164:5015. Vijayakrishnan, L. et al. (2004) Immunity 20:563. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401. Jansson, A. et al. (2005) J. Immunol 175:1575. Darlington, P.J. et al. (2005) J. Immunol. 175:996. Platt, A.M. et al. (2010) J. Immunol. 185:1558. Wing, K. et al. (2008) Science 322:271. Friedline, R.H. et al. (2009) J. Exp. Med. 206:421. Jain, N. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1524.
Long Name	Cytotoxic T-lymphocyte-associated Molecule 4
Entrez Gene IDs	1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)
Alternate Names	CD, CD152, CD152 antigen, CD152IDDM12, CD28, CELIAC3, CTLA-4, CTLA4, Cytotoxic T-lymphocyte-associated antigen 4, GRD4, GSE, ICOS, celiac disease 3, cytotoxic T-lymphocyte antigen 4, cytotoxic T-lymphocyte protein 4, cytotoxic T-lymphocyte-associated protein 4, cytotoxic T-lymphocyte-associated serine esterase-4, ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Citations for Recombinant Mouse CTLA-4 Fc Chimera Protein (12)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse CTLA-4 Fc Chimera Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

J Morimoto et al. (2022), Aire suppresses CTLA-4 expression from the thymic stroma to control autoimmunity Cell Reports, 38(7):110384.
PMID: 35172142
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Hanson et al. (2020), ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models PLoS ONE, 15(9):e0239595.
PMID: 32970735
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Kowalczyk A et al. (2014), Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3. Eur J Immunol, 44(4):1143-55.
PMID: 24338929
Species: Mouse
Sample Types: Serum
Applications: ELISA (Standard)

Sandin L et al. (2014), Local CTLA4 blockade effectively restrains experimental pancreatic adenocarcinoma growth in vivo. Oncoimmunology, 3(1):e27614.
PMID: 24701377
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Stojanovic A et al. (2014), CTLA-4 is expressed by activated mouse NK cells and inhibits NK Cell IFN-gamma production in response to mature dendritic cells. J Immunol, 192(9):4184-91.
PMID: 24688023
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Leitner et al. (2013), TIM-3 does not act as a receptor for galectin-9. PLoS Pathog, 9(3):e1003253.
PMID: 23555261
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Khan A et al. (2013), Dendritic cell modification as a route to inhibiting corneal graft rejection by the indirect pathway of allorecognition. Eur J Immunol, 43(3):734-46.
PMID: 23212959
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Peng et al. (2013), CD86 is an activation receptor for NK cell cytotoxicity against tumor cells. PLoS ONE, 8(12):e83913.
PMID: 24349559
Species: N/A
Sample Types: N/A
Applications: Bioassay

Butte MJ et al. (2007), Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses. Immunity, 27(1):111-22.
PMID: 17629517
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Anderson KM et al. (2006), Induction of CTLA-4-mediated anergy contributes to persistent colonization in the murine model of gastric Helicobacter pylori infection. J. Immunol., 176(9):5306-13.
PMID: 16621997
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

La Cava A et al. (2004), Ig-reactive CD4+CD25+ T cells from tolerized (New Zealand Black x New Zealand White)F1 mice suppress in vitro production of antibodies to DNA. J. Immunol., 173(5):3542-8.
PMID: 15322219
Species: Mouse
Sample Types: Serum
Applications: ELISA (Standard)

Bonham CA et al. (2002), Marked prolongation of cardiac allograft survival by dendritic cells genetically engineered with NF-kappa B oligodeoxyribonucleotide decoys and adenoviral vectors encoding CTLA4-Ig. , 169(6):3382-91.
PMID: 12218160

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FAQs for Recombinant Mouse CTLA-4 Fc Chimera Protein

Can Recombinant Mouse CTLA-4 Fc Chimera Protein (Catalog # 434-CT) be used with human cell lines or any other species?

The activity of Recombinant Mouse CTLA-4 Fc Chimera Protein (Catalog # 434-CT) is measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 0.1-0.4 µg/mL when stimulated with 1 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) in the presence of PHA. The product shows activity on the human Jurkat cell line. For human assay systems, please also consider Recombinant Human CTLA-4 Fc Chimera His-tag Protein (Catalog # 325-CT) or Recombinant Human CTLA-4 Fc Chimera (Catalog #7268-CT). Please refer the Citations tab to find publications reporting the use of the protein on other species. Filter the results based on species and sample type for a more relevant search to your target species.

Recombinant Mouse CTLA-4 Fc Chimera Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Product Specifications for Recombinant Mouse CTLA-4 Fc Chimera Protein

Scientific Data Examples for Recombinant Mouse CTLA-4 Fc Chimera Protein

Formulation, Preparation and Storage

Carrier Free

434-CT

Carrier

434-CT/CF

Carrier Free

Reconstitution Calculator

Background: CTLA-4

Citations for Recombinant Mouse CTLA-4 Fc Chimera Protein (12)

Customer Reviews for Recombinant Mouse CTLA-4 Fc Chimera Protein (1)

Recombinant Mouse CTLA-4 Fc Chimera Protein

FAQs for Recombinant Mouse CTLA-4 Fc Chimera Protein

Product Documents for Recombinant Mouse CTLA-4 Fc Chimera Protein

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