Recombinant Mouse COCO Protein

Name: Recombinant Mouse COCO Protein
Brand: R&D Systems
SKU: 3356-CC
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3356-CC

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Key Product Details

Source:	E. coli
Accession #:	Q76LW6
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse COCO Protein

Source	E. coli-derived mouse COCO protein Arg24-Leu185
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Arg24
Predicted Molecular Mass	17.3 kDa (monomer)
Activity	Measured by its ability to inhibit BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 0.6-3 µg/mL in the presence of 50 ng/mL of Recombinant Human BMP‑4 (Catalog # 314-BP).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3356-CC

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3356-CC/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: COCO

COCO, also known as DAND5, Dante, and CKTSF1B3, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and Cerberus (DAND4). DAN family members contain a cysteine knot domain that is homologous to that found in other TGF-beta superfamily ligands such as BMPs that play important roles in tissue morphogenesis and developmental processes (1-6). The mouse COCO cDNA encodes a 185 amino acid (aa) precursor with a 23 aa signal sequence (7, 8). COCO has eight Cys residues in the cysteine knot which places it in the CAN (or eight-membered ring) subfamily of BMP antagonists along with the other DAN family proteins (1). Mature mouse COCO shares 62% and 27% aa sequence identity with human and Xenopus COCO, respectively. It shares 22%-27% aa sequence identity with mouse DAN, Gremlin, PRDC, and Cerberus. In Xenopus embryos, COCO is expressed by pluripotent ectodermal cells. Expression is abruptly downregulated prior to gastrulation, and the loss of ectodermal cell pluripotency is coincident with COCO downregulation (7). COCO is required for Xenopus left-right axis formation (9). It functions predominantly on the right side of the embryo, although it is equally expressed on both left and right sides (9). COCO binds and inhibits activin, BMP-4, GDF-3/derrière, Wnt8, and Xnr1 (7, 9). In mouse, COCO expression is elevated on the right side of Henson’s node at the early somite stage, in contrast to the left side expression of Nodal (8).

Item	Value
References	Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1. Katoh, M. and M. Katoh (2004) Oncol. Rep. 12:423. Katoh, M. and M. Katoh (2005) Int. J. Mol. Med. 15:885. Baron, M.H. (2005) Exp. Hematol. 33:1015. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265. De Robertis, E.M. and H. Koruda (2004) Annu. Rev. Cell Dev. Biol. 20:285. Bell, E. et al. (2003) Development 130:1381. Pearce, J.J. et al. (1999) Dev. Biol. 209:98. Vonica, A. and A.H. Brivanlou (2007) Dev. Biol. 303:281.
Entrez Gene IDs	199699 (Human); 23863 (Mouse); 685719 (Rat)
Alternate Names	CER2CRL2, CERL2, CKTSF1B3, CKTSF1B3MGC126849, COCO, Cerberus 2, Cerberus like-2, Cerberus-like protein 2, Cysteine knot superfamily 1, BMP antagonist 3, DAN domain family member 5, DAN domain family, member 5, DAND5, DTE, Dante, FLJ38607, GREM3, GREM3cerberus 2, SP1, cerberus-like 2, cerl-2, gremlin-3

Citations for Recombinant Mouse COCO Protein (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse COCO Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Khalili et al. (2018), Induction of rod versus cone photoreceptor-specific progenitors from retinal precursor cells Stem Cell Res, 33(0):215-227.
PMID: 30453152

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Recombinant Mouse COCO Protein

Name: Saeed Khalili

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 11/16/2016

We use COCO in stem cell differentiation toward neural fate. The attached image is the ES-derived cone photoreceptor stained with cone arrestin.

Recombinant Mouse COCO Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse COCO Protein

Formulation, Preparation and Storage

Carrier Free

3356-CC

Carrier

3356-CC/CF

Carrier Free

Reconstitution Calculator

Background: COCO

Citations for Recombinant Mouse COCO Protein (1)

Customer Reviews for Recombinant Mouse COCO Protein (1)

Recombinant Mouse COCO Protein

Product Documents for Recombinant Mouse COCO Protein

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