Product Specifications for Recombinant Mouse CDO His-tag Protein, CF
Mouse myeloma cell line, NS0-derived mouse CDO protein Asp25-Tyr962, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
113-135 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Sonic Hedgehog/Shh (C25II), N-Terminus (Catalog # 464-SH) is immobilized at 4 µg/mL (100 µL/well), Recombinant Mouse CDO His-tag (Catalog # 10505-CD) binds with an ED50 of 0.8-8 ng/mL.
Scientific Data Examples for Recombinant Mouse CDO His-tag Protein, CF
Recombinant Mouse CDO His-tag Protein Binding Activity.
When Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus (464-SH) is immobilized at 4 µg/mL (100 µL/well), Recombinant Mouse CDO His-tag (Catalog # 10505-CD) binds with an ED50 of 0.8-8 ng/mL.
Recombinant Mouse CDO His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Mouse CDO His-tag Protein (10505-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 113-135 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
CAM-related/down-regulated by oncogene (CDO), also known as CDON is a member of the Ig/Fibronectin (FN) type III repeat family within the Ig superfamily. Mouse CDO is a type I transmembrane protein, consisting of a large extracellular domain (ECD), a transmembrane segment and a cytoplasmic region. The ECD contains five C2-type Ig-like domains, followed by three FN type III repeats (1). The first FN repeat is known to bind numerous cadherins, while the third (or juxtramembrane) FN type III repeat binds SHH (2, 3). The mature ECD of mouse CDO shares 85% amino acid identity to the ECD of human CDO. CDO is found on muscle precursor and neural progenitor cells of the embryo (4-6). It likely promotes muscle differentiation and contributes to axon guidance and neuronal patterning by signaling through various bHLH transcription factors (2, 5). These effects may be mediated through two different receptor complexes. On muscle precursors, CDO apparently acts as both a coordinating and signaling subunit. Here, it integrates N- and M-cadherin, neogenin, netrin-3 and BOC into a cis-oriented receptor complex (4, 7). While this complex has no identified ligand, intercellular cadherin interactions or netrin, may be enough to trigger CDO/cadherin/neogenin signaling. On axons, CDO may participate in a poorly-defined receptor complex minimally composed of CDO, BOC and Gas1 that binds SHH, and interacts with PTCH1 (8-10).
Kang, J.S. et al. (1997) J. Cell Biol. 138:203.
Yao, S. et al. (2006) Cell 125:343.
Kang, J-S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3989.
Kang, J-S. et al. (2002) EMBO J. 21:114.
Zhang, W. et al. (2006) Mol. Cell. Biol. 26:3764.
Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355.
Kang, J-S. et al. (2004) J. Cell. Biol. 167:493.
Okada, A. et al. (2006) Nature 444:369.
Allen, B.L. et al. (2007) Genes Dev. 21:1244.
Tenzen, T. et al. (2006) Dev. Cell 10:647.
Cell Adhesion Molecule-related/Down-regulated by Oncogenes
Entrez Gene IDs
50937 (Human); 57810 (Mouse)
CDO, CDON, CDOORCAM, Cdon homolog (mouse), MGC111524, ORCAM, cell adhesion molecule-related/down-regulated by oncogenes, surface glycoprotein, Ig superfamily member
Citations for Recombinant Mouse CDO His-tag Protein, CF
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Product Documents for Recombinant Mouse CDO His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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