Where does Cathepsin A cleave Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate, Catalog # ES005?

Name: Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF
Brand: R&D Systems
SKU: 9789-SE

Although the QC assay conditions provided on our recombinant Cathepsin A datasheets should favor carboxypeptidase activity, it is possible that there is more than one site in the ES005 peptide recognized and cleaved by Cathepsin A. We have not performed verification experiments to confirm the cleavage site preferred in our reaction conditions.

Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9789-SE

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Source:	NS0
Accession #:	P16675
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF

Product Specifications for Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF

Source	Mouse myeloma cell line, NS0-derived mouse Cathepsin A/Lysosomal Carboxypeptidase A protein Ala24-Tyr474, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala24
Predicted Molecular Mass	53 kDa
SDS-PAGE	52-60 kDa, reducing conditions
Activity	Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005). The specific activity is >160 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9789-SE

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

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Background: Cathepsin A/Lysosomal Carboxypeptidase A

Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1). Cathepsin A, also known as protective protein, is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds. Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH (2, 3). Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including endothelin and bradykinin making it a promising target in heart failure (2-5). A hereditary variant of cathepsin A results in cathepsin A-related arteriopathy with strokes and leukoencephalapathy (CARASAL) (6). In addition, Cathepsin A's association with beta -galactosidase ( beta -gal) and neuraminidase in a complex is essential for beta -gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of Cathepsin A causes Galactosialidosis, a lysosomal storage disorder, characterized by a combined secondary deficiency of beta -gal and neuraminidase (7, 8).

Item	Value
References	Pshezhetsky, A.V. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 1923, Academic Press, San Diego. Hiraiwa, M. (1999) Cell. Mol. Life. Sci. 56:894. Schreuder, H.A. et al. (2014) Biochem. Biophys. Res. Commun. 445:451. Ruf, S. et al. (2013) Future Med. Chem. 5:399. Timur, Z.K. et al. (2016) Front. Mol. Biosci. 3:68. Bugiani, M. et al. (2016) Neurology 84:1777. Caciotti, A. et al. (2013) Ophanet. J. Rare Dis. 8:114. Annunziata, I. et al. (2017) Expert Opin. Orphan Drugs 5:131.
Entrez Gene IDs	5476 (Human); 19025 (Mouse)
Alternate Names	CTSA, Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, EC 3.4.16, EC 3.4.16.5, GLB2, GSL, Lysosomal Carboxypeptidase A, PPCA, PPGBprotective protein for beta-galactosidase (galactosialidosis), Protective protein cathepsin A, Protective protein for beta-galactosidase, beta-galactosidase 2, beta-galactosidase protective protein, cathepsin ANGBE, lysosomal protective protein

Citations for Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF

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FAQs for Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF

Where does Cathepsin A cleave Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate, Catalog # ES005?

Although the QC assay conditions provided on our recombinant Cathepsin A datasheets should favor carboxypeptidase activity, it is possible that there is more than one site in the ES005 peptide recognized and cleaved by Cathepsin A. We have not performed verification experiments to confirm the cleavage site preferred in our reaction conditions.

Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Cathepsin A/Lysosomal Carboxypeptidase A

Citations for Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF

FAQs for Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF

Product Documents for Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF

Product Datasheets

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Certificate of Analysis

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