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Recombinant Mouse BTNL10/Butyrophilin-like 10 Fc Protein, CF

Catalog # 1444-BT | R&D Systems, Inc. a Bio-Techne Brand
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1444-BT-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BTNL10 protein
Mouse BTNL10
(Lys30-Glu167)
Accession # Q9JK39
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys30

Predicted Molecular Mass

42.6 kDa

SDS-PAGE

58-63 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for this effect is 0.5-2.5 μg/mL.

Scientific Data Images for Recombinant Mouse BTNL10/Butyrophilin-like 10 Fc Protein, CF

Recombinant Mouse BTNL10/Butyrophilin-like 10 Fc Protein Bioactivity

Recombinant Mouse BTNL10/Butyrophilin-like 10 Fc Protein Bioactivity

Recombinant Mouse BTNL10 Fc Chimera (Catalog # 1444-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 0.5-2.5 μg/mL.
Recombinant Mouse BTNL10/Butyrophilin-like 10 Fc Protein SDS-PAGE

Recombinant Mouse BTNL10/Butyrophilin-like 10 Fc Protein SDS-PAGE

2 μg/lane of Recombinant Mouse BTNL10 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58 - 63 kDa and 120 - 130 kDa, respectively.

Formulation, Preparation and Storage

1444-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: BTNL10

Butyrophilin-like 10 (BTNL10) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Mouse BTNL10 is a 275 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region, and a short cytoplasmic domain. The ECD of mouse BTNL10 features a single IgV and a poorly defined IgC domain. The IgV domain of mouse BTNL10 shares 53% and 52% sequence identity with the equivalent domain in human and rat BTNL10, respectively. Although recently identified, BTNL10 is one of only five butyrophilins conserved between human and mouse (2). While the complete immunological function of BTN/BTNL molecules is only beginning to emerge, they have been shown to be important in immunity by regulating T cell function (2-4). Recent efforts have focused on BTN/BTNL as potential therapeutic targets for a wide range of diseases (2-4). Currently, both the expression pattern and native function of BTNL10 remain unknown. Our data indicate that BTNL10 inhibits the human T cell activation, including IL-2, IFN-gamma secretion, and T cell proliferation.

References

  1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
  2. Arnett, H.A. et al. (2012) Immunogenetics. 64:781.
  3. Abeler-Dörner L. et al. (2012) Trends Immunol. 33(1):34.
  4. Arnett, H.A. and Viney J.L. (2014) Nat Rev Immunol. 14(8):559.

Long Name

Butyrophilin-like 10

Alternate Names

BTN4, BUTR1

Entrez Gene IDs

100129094 (Human); 192194 (Mouse)

Gene Symbol

BTNL10

UniProt

Additional BTNL10 Products

Product Documents for Recombinant Mouse BTNL10/Butyrophilin-like 10 Fc Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse BTNL10/Butyrophilin-like 10 Fc Protein, CF

For research use only

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