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Recombinant Mouse BAFFR/TNFRSF13C Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1357-BR

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1357-BR-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BAFF R/TNFRSF13C protein
Mouse BAFF R
(Ser10-Ala 71)
Accession # Q9D8D0
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser10

Predicted Molecular Mass

33 kDa (monomer)

SDS-PAGE

50-55 kDa, reducing conditions

Activity

Measured by its ability to inhibit BAFF-mediated proliferation of anti-IgM stimulated mouse B cells. Gross, J.A. et al. (2000) Nature 404:995.
The ED50 for this effect is 2-6 ng/mL in the presence of 1 ng/mL of Recombinant Mouse BAFF R/TNFRSF13C Fc Chimera
(Catalog # 8876-BF).

Formulation, Preparation and Storage

1357-BR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: BAFFR/TNFRSF13C

B-cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. It is produced by macrophages, dendritic cells, and T lymphocytes. BAFF promotes the survival of B cells and is essential for B cell maturation (1 - 4). BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL(a proliferation-inducing ligand), BAFF-R selectively binds BAFF. Mouse BAFF-R cDNA encodes a 175 amino acid residue (aa) transmembrane protein with a 71 aa extracellular domain, a 21 aa transmembrane domain, and a 83 aa cytoplasmic region. A second isoform of BAFF R that has a 72 aa cytoplamic region can also be produced by alternative splicing. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cells, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells and has profound defects in antibody mediated immune responses. Similarly, A/WySnJ mice that are defective in BAFF R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B-cell development. While the function of BCMA is not defined, TACI has been shown to control B-cell homeostasis and T-cell-independent immune responses.

References

  1. Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
  2. Mackay, F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
  3. Laabi, Y. et al. (2001) Current Biol. 11:R1013.
  4. Thompson, J.S. et al. (2001) Science 14:2108.

Long Name

B cell Activating Factor Receptor

Alternate Names

BAFF R, BR3, CD268, TNFRSF13C

Entrez Gene IDs

115650 (Human); 72049 (Mouse)

Gene Symbol

TNFRSF13C

UniProt

Additional BAFFR/TNFRSF13C Products

Product Documents for Recombinant Mouse BAFFR/TNFRSF13C Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse BAFFR/TNFRSF13C Fc Chimera Protein, CF

For research use only

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