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Recombinant Mouse B7-H3 Fc Chimera Protein, CF
Catalog # 10946-B3 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Accession #
Source
NS0
Structure / Form
Disulfide-linked
homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse B7-H3 protein
| Mouse B7-H3 (Val29-Phe244) Accession # Q8VE98.1 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val29
Predicted Molecular Mass
50 kDa
SDS-PAGE
65-73 kDa, under reducing conditions
Activity
Measured by its ability to inhibit Mouse CD3 epsilon Antibody induced proliferation of Mouse T cells. The ED50 for this effect is 0.06-0.72 µg/mL.
Scientific Data Images for Recombinant Mouse B7-H3 Fc Chimera Protein, CF
Recombinant Mouse B7‑H3 Fc Chimera Protein Bioactivity.
Recombinant Mouse B7-H3 Fc Chimera (Catalog # 10946-B3) inhibits Mouse CD3 epsilon Antibody (Clone # 145-2C11, MAB484) induced proliferation of Mouse T cells. The ED50 for this effect is 0.06-0.72 µg/mL.
Recombinant Mouse B7‑H3 Fc Chimera Protein SDS-PAGE.
2 μg/lane of Recombinant Mouse B7‑H3 Fc Chimera Protein (Catalog # 10946-B3) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-73 kDa and 130-150 kDa, respectively.Formulation, Preparation and Storage
10946-B3
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: B7-H3
T cells require a signal induced by the engagement of the T cell receptor and a “costimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with costimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3, and PD-L2 (1). B7-H3 is expressed at very high levels in immature dendritic cells at moderate levels on mature dendritic cells, LPS stimulated immature dendritic cells and LPS stimulated monocytes, and at low levels on resting monocytes. B7-H3 binds to activated T cells via an as-of-yet identified receptor. B7-H3 co-stimulates proliferation of T cells and interferon-gamma (IFN-gamma) production and enhances the induction of cytotoxic T cells. B7-H3 shares 20 - 27% amino acid (aa) identity with other B7 family members (2). Murine B7-H3 is a 259 aa protein containing an extracellular domain, a transmembrane domain and a cytoplasmic domain. Mouse and human B7-H3 share 87% aa identity (3).
References
- Coyle, A.J. and J.-C. Gutierrez-Ramos (2001) Nature Immunol. 2:203.
- Chapoval, A.I. et al. (2001) Nature Immunol. 2:269.
- Sun, M. et al. (2002) J. Immunol. 168:6294.
Long Name
B7 Homolog 3
Alternate Names
B7H3, CD276
Gene Symbol
CD276
UniProt
Product Documents for Recombinant Mouse B7-H3 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse B7-H3 Fc Chimera Protein, CF
For research use only
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