Recombinant Mouse ALCAM Fc Chimera Protein, CF

Name: Recombinant Mouse ALCAM Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1172-AL
Price: 394 USD

Catalog # 1172-AL | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	AAC06342
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse ALCAM Fc Chimera Protein, CF

Product Specifications for Recombinant Mouse ALCAM Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse ALCAM/CD166 protein

Mouse ALCAM (Trp28-Lys527) Accession # AAC06342	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Trp28

Predicted Molecular Mass

82 kDa (monomer)

SDS-PAGE

114-122 kDa, reducing conditions

Activity

Measured by its ability to block adhesion of HuT 78 human cutaneous T cell lymphoma cells to immobilized Recombinant Human CD6 Fc Chimera (Catalog # 627-CD). Recombinant Mouse ALCAM/CD166 Fc Chimera at 10 µg/mL (50 µL/well) blocked >90% of HUT-78 cell adhesion (1 x 10⁶ cells/mL, 50 µL/well) to immobilized recombinant human CD6 Fc Chimera (10 µg/mL, 100 µL/well).
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1172-AL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: ALCAM/CD166

ALCAM (activated leukocyte cell adhesion molecule), designated CD166 and also called MEMD and SC‑1/DM‑GRASP/BEN in the chicken, is a 100 ‑ 110 kDa type I transmembrane glycoprotein and a member of the Ig CAM family within the immunoglobulin superfamily (1). ALCAM is expressed on thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and monocyte‑derived dendritic cells (1, 2). Mouse ALCAM cDNA encodes 583 amino acid (aa), including signal peptide (27 aa), extracellular domain (ECD, 500 aa) with two V‑type and three C2‑type Ig‑like domains, transmembrane (22 aa) and cytoplasmic (34 aa) domains (1). Mouse ALCAM ECD shares 98%, 93% and 92% aa sequence identity with rat, human/porcine and bovine/equine ALCAM, respectively. A secreted isoform in endothelial cells that is truncated at aa 133 (sALCAM) antagonizes full‑length ALCAM (3, 4). ALCAM mediates low‑affinity adhesion with itself or the cysteine‑rich scavenger receptor CD6 to regulate T cell development, immunological synapses (IS), and cell migration through endothelial junctions (1 ‑ 11). ALCAM on thymic epithelia mediates adhesion to CD6 on CD4⁺CD8⁺ T cells (6). Adhesion of ALCAM‑expressing antigen presenting cells and CD6‑expressing T cells stabilizes the early IS, while later it enhances CD3 effects on T cell proliferation, CD25 expression, and Th1 commitment (2, 7, 8). High ALCAM expression at the blood‑brain barrier in active multiple sclerosis, and its mouse model (EAE), promotes leukocyte migration to the brain (8, 9). High ALCAM expression on human melanoma cell lines appears to be pro‑metastatic, but anti‑metastatic activity has been reported in human breast cancer (3, 10, 11). ALCAM may influence expression or adhesion of the neuronal adhesion molecule NCAM‑L1, both in the developing retina and invasive melanoma (3, 12).

Item	Value
References	Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213. Zimmerman, A.W. et al. (2006) Blood 107:3212. van Kilsdonk, J.W.J. et al. (2008) Cancer Res. 68:3671. Ikeda, K. and T. Quertermous (2004) J. Biol. Chem. 279:55315. van Kempen, L.C. et al. (2001) J. Biol. Chem. 276:25783. Castro, M.A.A. et al. (2007) J. Immunol. 178:4351. Nair, P. et al. (2010) Clin. Exp. Immunol. 162:116. Masedunskas, A. et al. (2006) FEBS Lett. 580:2637. Cayrol, R. et al. (2008) Nat. Immunol. 9:137. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805. King, J.A. et al. (2010) Mol. Cancer 9:266. Buhusi, M. et al. (2009) J. Neurosci. 29:15630.
Long Name	Activated Leukocyte Cell Adhesion Molecule
Entrez Gene IDs	214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)
Alternate Names	ALCAM, CD166, CD166 antigen, MEMDMGC71733, activated leucocyte cell adhesion molecule, activated leukocyte cell adhesion moleculeFLJ38514

Citations for Recombinant Mouse ALCAM Fc Chimera Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse ALCAM Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay, Cell Culture

CR Bye et al. (2019-07-12), Axonal growth of midbrain dopamine neurons is modulated by the cell adhesion molecule ALCAM through trans-heterophilic interaction with L1Cam, ChL1 and Semaphorin J. Neurosci., 2019-00(0).
PMID: 31300520
Sample Types: Small Molecule
Applications: Bioassay

Y Gao et al. (2017-06-20), Dietary sugars, not lipids, drive hypothalamic inflammation Mol Metab, 2017-06(8):897-908.
PMID: 28752053
Sample Types: N/A
Applications: Western Blot

Wierzbicki A et al. (2008-11-01), Immunization with a mimotope of GD2 ganglioside induces CD8+ T cells that recognize cell adhesion molecules on tumor cells. J. Immunol., 2008-1181(9):6644-53.
PMID: 18941255

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Recombinant Mouse ALCAM Fc Chimera Protein, CF

Catalog # 1172-AL | R&D Systems, Inc. a Bio-Techne Brand

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