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Recombinant Human VEGF-B 167 Protein, CF Best Seller

Catalog # 751-VEB | R&D Systems, Inc. a Bio-Techne Brand
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751-VEB-025

Key Product Details

Source

E. coli

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human VEGF-B protein
Pro22-Arg188

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro22

Predicted Molecular Mass

19 kDa (monomer)

SDS-PAGE

19-22 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat Neuropilin‑1 Fc Chimera (Catalog # 566-N1) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human VEGF-B 167 (Catalog # 751-VEB) binds with an ED50 of 10-80 ng/mL.

Scientific Data Images for Recombinant Human VEGF-B 167 Protein, CF

Recombinant Human VEGF-B 167 Protein Binding Activity

Recombinant Human VEGF-B 167 Protein Binding Activity

When Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) is immobilized at 1 µg/mL (100 µL/well) Recombinant VEGF-B 167 (Catalog # 751-VEB) binds with an ED50 of 10-80 ng/mL.
Recombinant Human VEGF-B 167 Protein SDS-PAGE

Recombinant Human VEGF-B 167 Protein SDS-PAGE

1 μg/lane of Recombinant Human VEGF-B 167 (Catalog # 751-VEB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 19-22 kD and 40-45 kDa, respectively..

Formulation, Preparation and Storage

751-VEB
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in 4mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF-B

Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity (1, 2). Five mammalian members, including VEGF-A, -B, -C, -D and PlGF, have been identified. VEGF family members are disulfide-linked dimeric proteins that are important regulators of physiological and pathological vasculogenesis, angiogenesis and lymphangiogenesis. VEGF-B is expressed in most tissues, especially in heart, skeletal muscle and pancreas. In many tissues, VEGF-B is co-expressed and can heterodimerize with VEGF (3). By alternative splicing, two isoforms of mature VEGF-B containing 167 or 186 amino acid (aa) residues exist (3, 4). The two VEGF-B isoforms have identical amino-terminal cysteine-knot VEGF homology domains but the carboxyl end of VEGF-B167 differs from that of VEGF-B186 by the presence of a highly basic cysteine-rich heparin binding domain. Whereas VEGF-B186 is a secreted diffusible protein, VEGF-B167 is sequestered into the cell matrix after secretion. Both VEGF-B isoforms bind VEGF receptor 1 (VEGF R1), but not VEGF R2 or VEGF R3 (5). On endothelial cells, ligation of VEGF R1 by VEGF-B has been shown to regulate the expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1. VEGF-B167 and a proteolytically processed form of VEGF-B186 (VEGF-B127) also bind neuropilin-1 (NP-1), a type I transmembrane receptor for semaphorins/collapsins, ligands involved in neuron guidance (6). Besides VEGF-B, NP‑1 has been shown to bind PLGF-2, VEGF165 and VEGF R1 (6, 7). The many interactions of NP-1 with VEGF ligands and receptor suggests that NP-1 may function as a regulator of angiogenesis (7).

References

  1. Li, X. and U. Eriksson (2001) Int. J. Biochem Cell Biol. 33:421.
  2. Olofsson, B. et al. (1999) Curr. Opin. Biotechnol. 10:528.
  3. Olofsson, B. et al. (1996) Proc. Nat. Acad. Sci. USA 93:2576.
  4. Grimmond, S. et al. (1996) Benome Res. 6:124.
  5. Olofsson, B. et al. (1998) Proc. Nat. Acad. Sci. USA 95:11709.
  6. Makinen, T. et al. (1999) J. Biol. Chem. 274:21217.
  7. Fuh, G. et al. (2000) J. Biol. Chem. 275:26690.

Long Name

Vascular Endothelial Growth Factor B

Alternate Names

VEGFB

Entrez Gene IDs

7423 (Human); 22339 (Mouse)

Gene Symbol

VEGFB

UniProt

Product Documents for Recombinant Human VEGF-B 167 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human VEGF-B 167 Protein, CF

For research use only

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