Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

Name: Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF
Brand: R&D Systems
SKU: 938-VC

Catalog # 938-VC | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	P33151
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

Technical Data
Citations (4) / Reviews

Product Specifications for Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

Source

Mouse myeloma cell line, NS0-derived human VE-Cadherin protein

Human VE-Cadherin (Asp48-Gln593) Accession # P33151	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Asp48

Predicted Molecular Mass

89 kDa (monomer)

SDS-PAGE

89 kDa (monomer)

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED₅₀ for this effect is 40-160 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

938-VC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

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Desired Reconstitution Concentration

Background: VE-Cadherin

VE-Cadherin, also known as Cadherin-5 and CD144, is a member of the atypical/type II subgroup of Cadherin homophilic adhesion proteins (1). Mature human VE-Cadherin is a 125-135 kDa transmembrane glycoprotein that consistis of a 552 amino acid (aa) extracellular domain (ECD) with five Ca⁺⁺-binding Cadherin domains, a 21 aa transmembrane segment, and a 164 aa cytoplasmic domain (2, 3). Within the ECD, human VE-Cadherin shares approximately 75% aa sequence identity with mouse and rat VE-Cadherin. A 90 kDa portion of the VE-Cadherin ECD can be shed in a metalloproteinase-dependent mechanism and may function as a VE-Cadherin antagonist (4, 5). VE-Cadherin is expressed on the surface of vascular endothelial cells (EC) from early in embryogenesis through adulthood as well as in the placenta, on hematopoietic cell progenitors, and on a subpopulation of hematopoietic stem cells (6-8). It is a major protein component of adherens junctions between cells of the endothelium (9). VE-Cadherin supports the extension and stabilization of existing vascular sprouts (10). VE-Cadherin also regulates or is regulated by VEGF R2, type I and type II TGF-beta receptors, and other endothelial junction proteins such as JAM-C, Claudin-5, and N-Cadherin (11-15). Elevated levels of soluble VE-Cadherin are found in the serum of myocardial infarction, angina pectoris, rheumatoid arthritis, and colorectal cancer patients (16-18).

Item	Value
References	Giannotta, M. et al. (2013) Dev. Cell 26:441. Breviario, F. et al. (1995) Arterioscler. Throm. Vasc. Biol. 15:1229. Geyer, H. et al. (1999) Glycobiology 9:915. Herren, B. et al. (1998) Mol. Biol. Cell 9:1589. Li, H. et al. (2010) Cancer Gene Ther. 17:700. Breier, G. et al. (1996) Blood 87:630. Ema, M. et al. (2006) Blood 108:4018. Oberlin, E. et al. (2010) Blood 116:4444. Lampugnani, M.G. et al. (1992) J. Cell Biol. 118:1511. Perryn, E.D. et al. (2008) Dev. Biol. 313:545. Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223. Orlova, V.V. et al. (2006) J. Exp. Med. 203:2703. Taddei, A. et al. (2008) Nat. Cell Biol. 10:923. Luo, Y. and G.L. Radice (2005) J. Cell Biol. 169:29. Rudini, N. et al. (2008) EMBO J. 27:993. Soeki, T. et al. (2004) Circ. J. 68:1. Sulkowska, M. et al. (2006) Tumori 92:67. Sidibe, A. et al. (2012) Arthritis Rheum. 64:77.
Long Name	Vascular Endothelium Cadherin
Entrez Gene IDs	1003 (Human); 12562 (Mouse)
Alternate Names	7B4, 7B4 antigen, CD144, CD144 antigen, CDH5, Cadherin-5, FLJ17376, VE-Cadherin, VECadherin, Vascular endothelial cadherin, cadherin 5, type 2 (vascular endothelium), cadherin 5, type 2, VE-cadherin (vascular epithelium), endothelial-specific cadherin

Citations for Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF include:

Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

N Gioelli et al. (2022-07-20), Neuropilin 1 and its inhibitory ligand mini-tryptophanyl-tRNA synthetase inversely regulate VE-cadherin turnover and vascular permeability Nature Communications, 2022-013(1):4188.
PMID: 35858913
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zhang P et al. (2015-10-26), Mutant B-Raf(V600E) Promotes Melanoma Paracellular Transmigration by Inducing Thrombin-mediated Endothelial Junction Breakdown. J Biol Chem, 2015-1291(5):2087-106.
PMID: 26504080
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Dunne E et al. (2012-04-26), Cadherin 6 has a functional role in platelet aggregation and thrombus formation. Arterioscler. Thromb. Vasc. Biol., 2012-032(7):1724-31.
PMID: 22539596
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Li X et al. (2007-08-16), Lentiviral rescue of vascular endothelial growth factor receptor-2 expression in flk1-/- embryonic stem cells shows early priming of endothelial precursors. Stem Cells, 2007-025(12):2987-95.
PMID: 17702981

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Showing 1 - 4 of 4 citations Showing All

Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

Catalog # 938-VC | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: VE-Cadherin

Citations for Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF (4)

Product Documents for Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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