Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF New

Name: Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI1298
Price: 466 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AVI1298

Product Datasheet / COA / SDS

Biotinylated

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	Q9BZM5.1
Structure / Form:	Disulfide-linked homodimer Biotinylated via Avi-tag
Applications:	Bioactivity

View Full Technical Details for Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF

Product Specifications for Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human ULBP-2 protein

Human ULBP-2 (Gly26-Ser217) Accession # Q9BZM5.1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly26

Predicted Molecular Mass

50 kDa

SDS-PAGE

60-70 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299-NK) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ULBP-2 Fc Chimera Avi-tag (Catalog # AVI1298) binds with an ED₅₀ of 0.500-8.00 ng/mL.

Scientific Data Examples for Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF

	Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein Binding Activity. When Recombinant Human NKG2D/CD314 Fc Chimera Protein (1299-NK) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ULBP‑2 Fc Chimera Avi-tag Protein (Catalog # AVI1298) binds with an ED₅₀ of 0.500-8.00 ng/mL.
	Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein (Catalog # AVI1298) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-70 kDa and 120-140 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI1298

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ULBP-2

ULBP-2 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. The name ULBP-2 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-2 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-2 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Our Avi-tag Biotinylated Recombinant Human ULBP-2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Item	Value
References	Cosman, D. et al. (2001) Immunity 14:123. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428. Sutherland, C. et al. (2002) J. Immunol. 168:671. Steinle, A. et al. (2001) Immunogenetics 53:279. Sutherland, C. et al. (2001) Immunol. Rev. 181:185. Pende, D. et al. (2002) Cancer Res. 62:6178. Radosavljevic, M. et al. (2002) Genomics 79:114. NKG2D and its Ligands (2002) www.rndsystems.com.
Long Name	UL16 Binding Protein-2
Entrez Gene IDs	80328 (Human)
Alternate Names	ALCAN-alpha, N2DL-2, N2DL2, NKG2D ligand 2, NKG2DL2, RAET1H, RAET1HNKG2DL2, Retinoic acid early transcript 1H, UL16 binding protein 2, UL16-binding protein 2, ULBP-2, ULBP2, retinoic acid early transcript 1 H

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Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF New

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF

Scientific Data Examples for Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: ULBP-2

Citations for Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF

Product Documents for Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF

Product Datasheets

COA

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