Recombinant Human ULBP-1 Fc Chimera Protein, CF

Name: Recombinant Human ULBP-1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1380-UL
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1380-UL

Citations (6)

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Source:	Sf 21 (stably transfected)
Accession #:	Q9BZM6
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

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Product Specifications for Recombinant Human ULBP-1 Fc Chimera Protein, CF

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived human ULBP-1 protein

Human ULBP-1 (Gly26 - Pro215) Accession # Q9BZM6	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly26

Predicted Molecular Mass

48.8 kDa (monomer)

SDS-PAGE

55 kDa, reducing conditions

Activity

Measured by its ability to bind rhNKG2D/Fc Chimera in a functional ELISA.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1380-UL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: ULBP-1

ULBP-1 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-1 has also been described under the names RaeT1I (retinoic acid early transcript), ALCAN-beta, and NKG2DL1. The name ULBP-1 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-1 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30 - 95%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. They are anchored to the membrane via a GPI-linkage. ULBP-1 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. ULBP-1 is expressed on some tumor cells and has been implicated in tumor surveillance (1 - 8).

Item	Value
References	Cosman, D. et al. (2001) Immunity 14:123. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428. Sutherland, C. et al. (2002) J. Immunol. 168:671. Steinle, A. et al. (2001) Immunogenetics 53:279. Sutherland, C. et al. (2001) Immunol. Rev. 181:185 Pende, D. et al. (2002) Cancer Res. 62:6178. Radosavljevic, M. et al. (2002) Genomics 79:114 NKG2D and its Ligands, 2002, www.RnDSystems.com.
Long Name	UL16 Binding Protein-1
Entrez Gene IDs	80329 (Human); 77777 (Mouse)
Alternate Names	ALCAN-beta, MULT-1, N2DL-1, N2DL1, NKG2D ligand 1, NKG2DL, NKG2DL1, RAET1I, RAET1Ialcan-beta, Retinoic acid early transcript 1I, UL16 binding protein 1, UL16-binding protein 1, ULBP-1, ULBP1

Citations for Recombinant Human ULBP-1 Fc Chimera Protein, CF (6)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human ULBP-1 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA (Standard)

AB Raneros et al. (2017), Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition Oncotarget, 8(19):31959-31976.
PMID: 28404876
Applications: Bioassay

S Weil et al. (2017), Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma Front Immunol, 8(0):387.
PMID: 28443091
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Ibusuki A et al. (2014), NKG2D triggers cytotoxicity in murine epidermal gammadelta T cells via PI3K-dependent, Syk/ZAP70-independent signaling pathway. J Invest Dermatol, 134(2):396-404.
PMID: 23962808
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Nuckel H et al. (2010), The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia. Leukemia, 24(6):1152-9.
PMID: 20428196
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA (Capture)

Ho VT et al. (2009), Biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation. Proc. Natl. Acad. Sci. U.S.A., 106(37):15825-30.
PMID: 19717467
Diermayr S et al. (2007), NKG2D ligand expression in AML increases in response to HDAC inhibitor valproic acid and contributes to allorecognition by NK-cell lines with single KIR-HLA class I specificities. Blood, 111(3):1428-36.
PMID: 17993609

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Recombinant Human ULBP-1 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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