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Recombinant Human Tyro3/Dtk His-tag Protein, CF

Catalog # 10365-DK | R&D Systems, Inc. a Bio-Techne Brand
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10365-DK-100

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Tyro3/Dtk protein
Ala41-Ser428, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala41

Predicted Molecular Mass

42 kDa

SDS-PAGE

63-71 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Tyro3/Dtk His-tag (Catalog # 10365-DK) is immobilized at 0.5 µg/mL (100 µL/well), it binds to Recombinant Human Gas6 (Catalog # 885-GSB) with an ED50 of 0.075-0.45 μg/mL.

Scientific Data Images for Recombinant Human Tyro3/Dtk His-tag Protein, CF

Recombinant Human Tyro3/Dtk His-tag Protein Binding Activity

Recombinant Human Tyro3/Dtk His-tag Protein Binding Activity

When Recombinant Human Tyro3/Dtk His-tag Protein (Catalog # 10365-DK) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Gas6 (Catalog # 885-GSB) binds with an ED50 of 0.075-0.45 µg/mL.
Recombinant Human Tyro3/Dtk His-tag Protein SDS-PAGE

Recombinant Human Tyro3/Dtk His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Tyro3/Dtk His-tag (Catalog # 10365-DK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 63-71 kDa.

Formulation, Preparation and Storage

10365-DK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily (1, 2). The overall genomic structure of Dtk is virtually identical to that determined for the human UFO gene. This particular genomic organization is likely to have been duplicated and closely maintained throughout evolution (3). The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases (1). All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anti-coagulation factor protein S (1, 2). The binding affinities for Gas6 is in the order of Axl > Dtk > Mer (1). Gas6 binding induces tyrosine phosphorylation and down-stream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (1, 2). Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain (4). The full length of Human DTK is 428 amino acids, with 387 amino acid signal sequence in the mature extracellular domain. The ECD of human DTK has 7 glycosylation sites. Within the ECD, human DTK shares 85% sequence identity with both mouse and rat DTK.

References

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
  3. Paula, M. et al. (1996) Genomics 31:13.
  4. Philip, S. et al. (1994) Growth Factor 11:125.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Entrez Gene IDs

7301 (Human); 22174 (Mouse); 102135978 (Cynomolgus Monkey)

Gene Symbol

TYRO3

UniProt

Product Documents for Recombinant Human Tyro3/Dtk His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Tyro3/Dtk His-tag Protein, CF

For research use only

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