Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF

Name: Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 859-DK
Rating: 5 (1 reviews)

Catalog # 859-DK | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	Q06418
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

View Full Technical Details for Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF

Technical Data
Citations (2) / Reviews (1)

Product Specifications for Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human Tyro3/Dtk protein

Human Tyro3/Dtk (Ala41-Ser428) Accession # Q06418	DIEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala41

Predicted Molecular Mass

69 kDa (monomer)

SDS-PAGE

69 kDa (monomer)

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Tyro3/Dtk Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Human Gas6 (Catalog # 885-GSB) with a linear range of 2-100 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

859-DK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

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Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

Item	Value
References	Nagata, K. et al. (1996) J. Biol. Chem. 22:30022. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name	Tyrosine Protein Kinase Receptor TYRO3
Entrez Gene IDs	7301 (Human); 22174 (Mouse)
Alternate Names	BYK, Brt, Dtk, EC 2.7.10, EC 2.7.10.1, FLJ16467, Rse, Sky, TYRO3 protein tyrosine kinase, Tif, Tyro3, Tyrosine-protein kinase DTK, Tyrosine-protein kinase RSE, Tyrosine-protein kinase SKY, Tyrosine-protein kinase byk, tyrosine-protein kinase receptor TYRO3

Citations for Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cerchia et al. (2012-08-21), Targeting Axl with an high-affinity inhibitory aptamer. Mol Ther, 2012-020(12):2291-303.
PMID: 22910292
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay

Shimojima M et al. (2011-12-07), Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry. J. Virol., 2011-186(4):2067-78.
PMID: 22156524

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Customer Reviews for Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF (1)

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Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 04/03/2018

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Product Documents for Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF

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Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF

Catalog # 859-DK | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Tyro3/Dtk

Citations for Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF (2)

Customer Reviews for Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF (1)

Customer Images

Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF

Product Documents for Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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