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Recombinant Human TREM-1 Fc Chimera Avi-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # AVI1278

Biotinylated
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AVI1278-050

Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TREM-1 protein
Human TREM-1
(Ala21-Arg200)
Accession # Q9NP99.1
IEGRMD Human IgG1
(Pro100-Lys330)
Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21

Predicted Molecular Mass

49 kDa

SDS-PAGE

60-78 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PGLYRP1/PGRP-S Protein (Catalog # 2590-PGB) is immobilized at 1 μg/mL (100 μL well), Biotinylated Recombinant Human TREM‑1 Fc Chimera Avi-tag (Catalog # AVI1278) binds with an ED50 of 15.0-150 ng/mL.

Scientific Data Images for Recombinant Human TREM-1 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human TREM‑1 Fc Chimera Avi-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Human PGLYRP1/PGRP-S Protein (2590-PGB) is immobilized at 1 μg/mL (100 μL well), Biotinylated Recombinant Human TREM‑1 Fc Chimera Avi-tag Protein (Catalog # AVI1278) binds with an ED50 of 15.0‑150 ng/mL.

Biotinylated Recombinant Human TREM‑1 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human TREM‑1 Fc Chimera Avi-tag Protein (Catalog # AVI1278) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-78 kDa and 110-150 kDa, respectively.

Formulation, Preparation and Storage

AVI1278
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1 and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS‑induced shock. Blockade of TREM-1 with a TREM‑1/Fc fusion protein protected mice against LPS‑induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1 - 3). Our Avi-tag Biotinylated human TREM-1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.

Long Name

Triggering Receptor Expressed on Myeloid Cells 1

Alternate Names

CD354, TREM1

Entrez Gene IDs

54210 (Human); 58217 (Mouse)

Gene Symbol

TREM1

UniProt

Additional TREM-1 Products

Product Documents for Recombinant Human TREM-1 Fc Chimera Avi-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human TREM-1 Fc Chimera Avi-tag Protein, CF

For research use only

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