Recombinant Human PGLYRP1/PGRP-S Protein, CF (catalog # 2590-PGB) is lyophilized from a 0.2 μm filtered solution in MOPS and NaCl. What happens to the MOPS during lyophilization?

Name: Recombinant Human PGLYRP1/PGRP-S Protein, CF
Brand: R&D Systems
SKU: 2590-PGB

MOPS is commercially available as a crystalline solid. During the lyophilization process, the water in the buffer evaporates, leaving behind crystalline MOPS and NaCl residues.

Recombinant Human PGLYRP1/PGRP-S Protein, CF

Catalog # 2590-PGB | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	O75594
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human PGLYRP1/PGRP-S Protein, CF

Technical Data
Citations (2) / Reviews / FAQs

Product Specifications for Recombinant Human PGLYRP1/PGRP-S Protein, CF

Source	Mouse myeloma cell line, NS0-derived human PGLYRP1/PGRP-S protein Gln22-Pro196, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained. Gln22 predicted
Predicted Molecular Mass	20 kDa
SDS-PAGE	20 kDa
Activity	Measured by its binding ability in a functional ELISA. When peptidoglycan is coated at 1 µg/mL (100 µL/well), the concentration of Recombinant Human PGLYRP1/PGRP-S that produces 50% optimal binding response is 0.75-4.5 ng/mL.

Scientific Data Examples for Recombinant Human PGLYRP1/PGRP-S Protein, CF

	Bioactivity of Recombinant Human PGLYRP1/PGRP-S protein When peptidoglycan is coated at 1 µg/mL, 100 µL/well, Recombinant Human PGLYRP1/PGRP-S binds with an ED₅₀ of 0.75-4.5 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2590-PGB

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PGLYRP1/PGRP-S

The human PGRP family is comprised of four peptidoglycan recognition proteins that may function as innate immunity pattern recognition molecules (1, 2). Termed PGRP-L, PGRP-I alpha, PGRP-I beta and PGRP-S, they are all products of separate genes, and all are named for the relative length of their translated product (3). PGRP-L (for long) is 576 amino acids (aa) in length, while PGRP-I alpha and I beta are (I) intermediate in length at 341 aa and 373 aa, respectively, and PGRP-S is the shortest at 196 aa in length (3, 4). All human PGRPs bind peptidoglycan and Gram-positive bacteria, and all have at least three C-terminal PGRP domains at variable sites that are highly conserved from insects to mammals (3). Human PGRP-S, the first described member of the family, is a 28 kDa secreted glycoprotein associated with neutrophils (4). The mature molecule is 175 aa in length and contains three variably-sized peptide-carbohydrate recognition sequences of 15 aa, 29 aa and 49 aa, respectively. Human PGRP-S is 72%, 71% and 70% aa identical to mouse, bovine and rat mature PGRP-S, respectively. Studies with PGRP-S deficient mice indicate that knock-out mice have increased susceptibility to infections with non-pathogenic bacteria. Neutrophils from knock-out mice exhibit normal phagocytosis of bacteria but are defective in intracellular killing and digestion of nonpathogenic bacteria (5). The longer three PGRP members are all membrane-bound molecules that contain two membrane-spanning segments. Both the N- and C-termini are depicted as being extracellular with a joining cytoplasmic domain. All three transmembrane forms show at least one PGRP domain on the C-terminal extracellular region; other PGRP domains are variably distributed over their two extracellular and one cytoplasmic region (3).

Item	Value
References	Girardin, S.E. and D.J. Philpott (2004) Eur. J. Immunol. 34:1777. Steiner, H. (2004) Immunol. Rev. 198:83. Liu, C. et al. (2001) J. Biol. Chem. 276:34686. Kang, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10078. Dziarski, R. et al. (2003) Blood 102:689.
Long Name	Peptidoglycan Recognition Protein Short/Peptidoglycan Recognition Protein 1
Entrez Gene IDs	8993 (Human); 21946 (Mouse)
Alternate Names	PGLYRP, PGLYRP1, PGRP-S, PGRP-SMGC126894, PGRPS, PGRPSMGC126896, PGRPpeptidoglycan recognition protein, Peptidoglycan recognition protein short, TAG7, TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein), TNFSF3L, peptidoglycan recognition protein 1

Citations for Recombinant Human PGLYRP1/PGRP-S Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human PGLYRP1/PGRP-S Protein, CF include:

Species: Borrelia burgdorferi (Lyme Disease)
Sample Types: Cell Lysates
Applications: ICC

A Gupta et al. (2020-11-11), A human secretome library screen reveals a role for Peptidoglycan Recognition Protein 1 in Lyme borreliosis PLoS Pathog, 2020-116(11):e1009030.
PMID: 33175909
Species: Human

Applications: Surface Plasmon Resonance

Read C et al. (2015-01-16), Cutting Edge: identification of neutrophil PGLYRP1 as a ligand for TREM-1. J Immunol, 2015-0194(4):1417-21.
PMID: 25595774

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FAQs for Recombinant Human PGLYRP1/PGRP-S Protein, CF

Recombinant Human PGLYRP1/PGRP-S Protein, CF (catalog # 2590-PGB) is lyophilized from a 0.2 μm filtered solution in MOPS and NaCl. What happens to the MOPS during lyophilization?

MOPS is commercially available as a crystalline solid. During the lyophilization process, the water in the buffer evaporates, leaving behind crystalline MOPS and NaCl residues.

Recombinant Human PGLYRP1/PGRP-S Protein, CF

Catalog # 2590-PGB | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human PGLYRP1/PGRP-S Protein, CF

Scientific Data Examples for Recombinant Human PGLYRP1/PGRP-S Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: PGLYRP1/PGRP-S

Citations for Recombinant Human PGLYRP1/PGRP-S Protein, CF (2)

FAQs for Recombinant Human PGLYRP1/PGRP-S Protein, CF

Product Documents for Recombinant Human PGLYRP1/PGRP-S Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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