Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis. It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

Can Recombinant Human TGF beta 1 Protein (Catalog # 7754-BH or 7754-BH/CF) be reconstituted in PBS or water?

Recombinant Human TGF beta 1 Protein (Catalog # 7754-BH or 7754-BH/CF) needs to be reconstituted to 100 μg/mL in sterile 4 mM HCl containing 0.1% BSA or 4 mM HCl. This protein is hydrophobic by nature, so it requires an acidic buffer for reconstitution to go completely into solution. Stability testing has been evaluated on the protein with these reconstitution conditions; we can guarantee performance under these reconstitution conditions. When the protein is diluted with the cell culture media or buffers to the working concentration, the concentration of the acid should be buffered or negligible.

Has the half-life of recombinant human TGF beta 1 been determined?

While our Recombinant Human TGF-beta 1 Protein has been evaluated for bioactivity, the half-life of a specific protein will depend on experimental conditions, including the cell number, density, and media content. It is up to the end-user to determine the appropriate concentration and timing when adding Recombinant Human TGF-beta 1 Protein to individual experiments. For techniques and methodologies, we recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein Best Seller

Name: Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
Brand: R&D Systems
SKU: 7754-BH
Rating: 5 (15 reviews)

Catalog # 7754-BH | R&D Systems, Inc. a Bio-Techne Brand

(15)

Citations (53)

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Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	P01137
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Product Specifications for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Source	Human embryonic kidney cell, HEK293-derived human TGF-beta 1 protein Ala279-Ser390
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala279
Predicted Molecular Mass	12.8 kDa (monomer)
SDS-PAGE	11 kDa, reducing conditions
Activity	Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED₅₀ for this effect is 0.04-0.2 ng/mL.

Scientific Data Examples for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

	Bioactivity of Human TGF-beta 1 Protein Recombinant human TGF-beta 1 (7754-BH) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED₅₀ for this effect is 0.04-0.2 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

7754-BH

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7754-BH/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 1

TGF‑ beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF‑ beta superfamily that share a characteristic cystine knot structure (1‑7). TGF‑ beta 1, ‑2 and ‑3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non‑redundant functions; for TGF‑ beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide-linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

Item	Value
References	Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press p. 29. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982. Wahl, S.M. (2006) Immunol. Rev. 213:213. Chang, H. et al. (2002) Endocr. Rev. 23:787. Lin, J.S. et al. (2006) Reproduction 132:179. Hinck, A.P. et al. (1996) Biochemistry 35:8517. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261. Derynck, R. et al. (1985) Nature 316:701. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name	Transforming Growth Factor beta 1
Entrez Gene IDs	7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names	CEDLAP, DPD1, TGF beta, TGF beta1, TGF-beta 1, TGF-beta 1 protein, TGF-beta-1, TGFB, TGFB1, TGFbeta, TGFbeta 1, latency-associated peptide, transforming growth factor beta-1, transforming growth factor, beta 1

Citations for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein (53)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

De Martin et al. (2023-05-18), PI16+ reticular cells in human palatine tonsils govern T cell activity in distinct subepithelial niches Nature immunology
PMID: 37202490
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

V Klück et al. (2023-02-27), TGF-beta is elevated in hyperuricemic individuals and mediates urate-induced hyperinflammatory phenotype in human mononuclear cells Arthritis Research Therapy, 2023-02-27;25(1):30.
PMID: 36850003
Species: Human, Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay

VMD Almanzar et al. (2023-02-16), 5-FU-miR-15a Inhibits Activation of Pancreatic Stellate Cells by Reducing YAP1 and BCL-2 Levels In Vitro International Journal of Molecular Sciences, 2023-024(4).
PMID: 36835366
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

WL Yang et al. (2023-02-16), Nsun2 coupling with RoRgammat shapes the fate of Th17 cells and promotes colitis Nature Communications, 2023-014(1):863.
PMID: 36792629
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

DI Cho et al. (2023-02-13), ANGPTL4 stabilizes atherosclerotic plaques and modulates the phenotypic transition of vascular smooth muscle cells through KLF4 downregulation Experimental Molecular Medicine, 2023-02-13;0(0).
PMID: 36782020
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

AH Mandarano et al. (2023-02-10), DRAK2 contributes to type 1 diabetes by negatively regulating IL-2 sensitivity to alter regulatory Tï¿½cell development Cell Reports, 2023-042(2):112106.
PMID: 36773294
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Migda? et al. (2023-01-11), xcore: an R package for inference of gene expression regulators BMC Bioinformatics, 2023-024(1):14.
PMID: 36631751
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

J Feng et al. (2022-12-21), TGF-beta signaling and Creb5 cooperatively regulate Fgf18 to control pharyngeal muscle development Elife, 2022-111(0).
PMID: 36542062
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

DQ Chen et al. (2022-12-05), Poricoic acid A suppresses renal fibroblast activation and interstitial fibrosis in UUO rats via upregulating Sirt3 and promoting beta-catenin K49 deacetylation Acta pharmacologica Sinica, 2022-10(0).
PMID: 36470978
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

D Cai et al. (2022-10-28), Nanoparticle endothelial delivery of PGC-1alpha attenuates hypoxia-induced pulmonary hypertension by attenuating EndoMT-caused vascular wall remodeling Redox Biology, 2022-158(0):102524.
PMID: 36334380
Species: Complex Species Category
Sample Types: Whole Cells
Applications: Bioassay

Bhuyan S et al. (2022-09-29), Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense. Front Immunol, 2022-013(0):933329.
PMID: 36248858
Species: Human
Sample Types: Organoid
Applications: Bioassay

B Jaeger et al. (2022-09-26), Airway basal cells show a dedifferentiated KRT17highPhenotype and promote fibrosis in idiopathic pulmonary fibrosis Nature Communications, 2022-013(1):5637.
PMID: 36163190
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

X Guo et al. (2022-09-23), LINC01146/F11R facilitates growth and metastasis of prostate cancer under the regulation of TGF-beta The Journal of steroid biochemistry and molecular biology, 2022-00(0):106193.
PMID: 36162632
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Gao et al. (2022-09-11), HDAC5-mediated Smad7 silencing through MEF2A is critical for fibroblast activation and hypertrophic scar formation International journal of biological sciences, 2022-018(15):5724-5739.
PMID: 36263180
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay

Kartasheva-Ebertz D et al. (2022-08-29), IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation. Int J Mol Sci, 2022-023(17).
PMID: 36077175
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Z Cai et al. (2022-07-19), Kynurenine metabolites predict survival in pulmonary arterial hypertension: A role for IL-6/IL-6Ralpha Scientific Reports, 2022-012(1):12326.
PMID: 35853948
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A El-Gazzar et al. (2022-07-16), SMAD3 mutation in LDS3 causes bone fragility by impairing the TGF-beta pathway and enhancing osteoclastogenesis Bone Reports, 2022-017(0):101603.
PMID: 35874167
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

RB Werder et al. (2022-07-13), CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells Oncogene, 2022-08(28):eabo6566.
PMID: 35857525
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay

MD Gundersen et al. (2022-07-07), Hypo-osmotic stress induces the epithelial alarmin IL-33 in the colonic barrier of ulcerative colitis Scientific Reports, 2022-012(1):11550.
PMID: 35798804
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Montefusco D et al. (2022-06-06), Sphingosine kinase 1 mediates sexual dimorphism in fibrosis in a mouse model of NASH. Mol Metab, 2022-062(0):101523.
PMID: 35671973
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Dai Y et al. (2022-06-01), Modified Shenlingbaizhu Decoction represses the pluripotency of colorectal cancer stem cells by inhibiting TGF-beta mediated EMT program. Phytomedicine, 2022-0103(0):154234.
PMID: 35689903
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JC Bachmann et al. (2022-05-17), Fibrotic Signaling in Cardiac Fibroblasts and Vascular Smooth Muscle Cells: The Dual Roles of Fibrosis in HFpEF and CAD Cells, 2022-011(10).
PMID: 35626694
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

B Liu et al. (2022-04-23), Proximal tubular RAGE mediated the renal fibrosis in UUO model mice via upregulation of autophagy Cell Death Disease, 2022-04-23;13(4):399.
PMID: 35461309
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

VT Luu et al. (2022-03-11), Dedifferentiation of Human Cardiac Myofibroblasts Is Independent of Activation of COX-2/PGE2 Pathway International Journal of Molecular Sciences, 2022-023(6).
PMID: 35328443
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SP Chapoval et al. (2022-03-11), Identifying Function Determining Residues in Neuroimmune Semaphorin 4A International Journal of Molecular Sciences, 2022-023(6).
PMID: 35328445
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Feng et al. (2022-03-10), Down-regulation of the endothelial histone demethylase JMJD3 is associated with neointimal hyperplasia of arteriovenous fistulas in kidney failure The Journal of Biological Chemistry, 2022-00(0):101816.
PMID: 35278430
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Guo et al. (2022-02-23), PD-L1 mediates lung fibroblast to myofibroblast transition through Smad3 and beta-catenin signaling pathways Scientific Reports, 2022-012(1):3053.
PMID: 35197539
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Sun et al. (2022-02-08), PD-L1 promotes myofibroblastic activation of hepatic stellate cells by distinct mechanisms selective for TGF-beta receptor I versus II Cell Reports, 2022-038(6):110349.
PMID: 35139382
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilks B et al. (2022-02-01), Quantifying Cell-Derived Changes in Collagen Synthesis, Alignment, and Mechanics in a 3D Connective Tissue Model. Adv Sci (Weinh), 2022-09(10):e2103939.
PMID: 35102708
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Liu Y et al. (2022-01-12), Bone marrow mesenchymal stem cell-derived exosomes improve renal fibrosis via regulating Smurf 2/Smad 7. Front Biosci (Landmark Ed), 2022-027(1):17.
PMID: 35090322
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Farrell E et al. (2021-11-20), Spatiotemporal control of myofibroblast activation in acoustically-responsive scaffolds via ultrasound-induced matrix stiffening. Acta Biomater, 2021-1138(0):133-143.
PMID: 34808418
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Suezawa et al. (2021-11-18), Disease modeling of pulmonary fibrosis using human pluripotent stem cell-derived alveolar organoids Stem Cell Reports, 2021-10(0).
PMID: 34798066
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Jin J et al. (2021-10-14), Lobeglitazone, A Peroxisome Proliferator-Activated Receptor-Gamma Agonist, Inhibits Papillary Thyroid Cancer Cell Migration and Invasion by Suppressing p38 MAPK Signaling Pathway. Endocrinol Metab (Seoul), 2021-136(5):1095-1110.
PMID: 34645125
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay

Kienzl M et al. (2021-09-11), Monoacylglycerol lipase deficiency in the tumor microenvironment slows tumor growth in non-small cell lung cancer. Oncoimmunology, 2021-010(1):1965319.
PMID: 34527428
Species: Human
Sample Types: Transduced Whole Cells
Applications: Bioassay

Ghilardi S et al. (2021-06-30), Ventral stress fibers induce plasma membrane deformation in human fibroblasts. Mol Biol Cell, 2021-032(18):1707-1723.
PMID: 34191528
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Marwick J et al. (2021-06-02), Application of a High-Content Screening Assay Utilizing Primary Human Lung Fibroblasts to Identify Antifibrotic Drugs for Rapid Repurposing in COVID-19 Patients. SLAS Discov, 2021-026(9):1091-1106.
PMID: 34078171
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Bsat M et al. (2021-03-01), The Conversion of Human Tissue-Like Inflammatory Monocytes Into Macrophages. Curr Protoc, 2021-01(3):e74.
PMID: 33705607
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Shen et al. (2020-11-04), Identification of metabolism-associated genes and construction of a prognostic signature in bladder cancer Cancer Cell International, 2020-120(1):538.
PMID: 33292266
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wu P et al. (2020-09-30), Tumor Cell-Derived TGFbeta1 Attenuates Antitumor Immune Activity of T Cells via Regulation of PD-1 mRNA. Cancer Immunol Res, 2020-08(12):1470-1484.
PMID: 32999004
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

R da Silva A et al. (2020-09-29), Lack of evidence supporting a role of IFN-&beta and TGF-&beta in differential polarization of Bordetella pertussis specific-T cell responses Cytokine, 2020-0137(0):155313.
PMID: 33002739
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

TJ Aikin et al. (2020-09-17), MAPK activity dynamics regulate non-cell autonomous effects of oncogene expression Elife, 2020-09(0).
PMID: 32940599
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

W Kong et al. (2020-03-16), Nucleolar protein NOP2/NSUN1 suppresses HIV-1 transcription and promotes viral latency by competing with Tat for TAR binding and methylation PLoS Pathog., 2020-016(3):e1008430.
PMID: 32176734
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Zhou et al. (2019-11-06), CD109 acts as a gatekeeper of the epithelial trait by suppressing epithelial to mesenchymal transition in squamous cell carcinoma cells in vitro Sci Rep, 2019-19(1):16317.
PMID: 31695056
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay

Islam S et al. (2019-10-26), Accumulation of versican facilitates wound healing: Implication of its initial ADAMTS-cleavage site. Matrix Biol, 2019-187(0):77-93.
PMID: 31669737
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Li Z et al. (2019-07-08), ERK Regulates HIF1alpha-Mediated Platinum Resistance by Directly Targeting PHD2 in Ovarian Cancer. Clin Cancer Res, 2019-025(19):5947-5960.
PMID: 31285371
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Arenas Cor et al. (2018-12-13), Ruxolitinib in combination with prednisone and nilotinib exhibit synergistic effects in human cells lines and primary cells from myeloproliferative neoplasms Haematologica, 2018-10(0).
PMID: 30545926
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

F Chen et al. (2018-08-23), Retinoic acid signaling is essential for airway smooth muscle homeostasis JCI Insight, 2018-03(16).
PMID: 30135301
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

B Kumar et al. (2018-07-11), Normal breast-derived epithelial cells with luminal and intrinsic subtype-enriched gene expression document inter-individual differences in their differentiation cascade Cancer Res., 2018-00(0).
PMID: 29997232
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

D Berner et al. (2017-11-01), Posttranscriptional Regulation of LOXL1 Expression Via Alternative Splicing and Nonsense-Mediated mRNA Decay as an Adaptive Stress Response Invest. Ophthalmol. Vis. Sci., 2017-158(13):5930-5940.
PMID: 29164236
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Huang et al. (2017-06-07), A Novel Bromodomain Inhibitor Reverses HIV-1 Latency through Specific Binding with BRD4 to Promote Tat and P-TEFb Association Front Microbiol, 2017-08(0):1035.
PMID: 28638377
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zhang H et al. (2017-05-26), The human RNA surveillance factor Up-frameshift 1 inhibits hepatic cancer progression by targeting MRP2/ABCC2. Biomed Pharmacother, 2017-092(0):365-372.
PMID: 28554132
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JL Meitzler et al. (2017-05-26), Decoding NADPH oxidase 4 expression in human tumors Redox Biol, 2017-013(0):182-195.
PMID: 28578276
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kim K et al. (2016-06-29), Glucocorticoids Have Opposing Effects on Liver Fibrosis in Hepatic Stellate and Immune Cells. Mol Endocrinol, 2016-030(8):905-16.
PMID: 27355192

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Customer Reviews for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein (15)

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Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Reason for Rating: We used 10nM of TGF-beta to treat quiescent cancer associated fibroblasts, to transform them into activated cancer associated fibroblasts, as an positive control.

Verified Customer | Posted 09/07/2023

We used several fibroblasts, to assess the immune cells interaction.
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Anonymous

Application: Apoptosis assay

Verified Customer | Posted 02/02/2023
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Anonymous

Application: CellProlif

Reason for Rating: I used it in a number of cells in several assays and always worked as expected.

Verified Customer | Posted 01/20/2023

Human liver cells were serum starved over night before TGF treatment.
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 11/18/2020
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Yuqing Feng

Application: In vitro bioactivity in cell culture

Reason for Rating: No discernible difference between HEK293-derived TGFb vs CHO-derived TGFb (cat#240-B) was found

Verified Customer | Posted 10/17/2019
Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 08/30/2019
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Jenna Nguyen

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 05/07/2019
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 02/10/2019

TGFB1 human peritoneal mesothelial cells
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Anonymous

Application: CellProlif

Verified Customer | Posted 05/22/2018
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Anonymous

Application: Cell migration/motility

Verified Customer | Posted 04/12/2018
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Anonymous

Application: CellProlif

Verified Customer | Posted 03/08/2018
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Anonymous

Application: Enzymatic activity in vitro

Verified Customer | Posted 03/07/2018
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Anonymous

Application: Apoptosis assay

Verified Customer | Posted 02/17/2018
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Anonymous

Application: CellProlif

Verified Customer | Posted 02/14/2018
Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 10/01/2017

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FAQs for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis. It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.
Can Recombinant Human TGF beta 1 Protein (Catalog # 7754-BH or 7754-BH/CF) be reconstituted in PBS or water?

Recombinant Human TGF beta 1 Protein (Catalog # 7754-BH or 7754-BH/CF) needs to be reconstituted to 100 μg/mL in sterile 4 mM HCl containing 0.1% BSA or 4 mM HCl. This protein is hydrophobic by nature, so it requires an acidic buffer for reconstitution to go completely into solution. Stability testing has been evaluated on the protein with these reconstitution conditions; we can guarantee performance under these reconstitution conditions. When the protein is diluted with the cell culture media or buffers to the working concentration, the concentration of the acid should be buffered or negligible.
Has the half-life of recombinant human TGF beta 1 been determined?

While our Recombinant Human TGF-beta 1 Protein has been evaluated for bioactivity, the half-life of a specific protein will depend on experimental conditions, including the cell number, density, and media content. It is up to the end-user to determine the appropriate concentration and timing when adding Recombinant Human TGF-beta 1 Protein to individual experiments. For techniques and methodologies, we recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein Best Seller

Catalog # 7754-BH | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Scientific Data Examples for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Formulation, Preparation and Storage

Carrier Free

7754-BH

Carrier

7754-BH/CF

Carrier Free

Reconstitution Calculator

Background: TGF-beta 1

Citations for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein (53)

Customer Reviews for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein (15)

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Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

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