Recombinant Human TGF-beta 1 GMP Protein, CFGMP

Name: Recombinant Human TGF-beta 1 GMP Protein, CF
Brand: rnd
SKU: 240-GMP

Catalog # 240-GMP

Citations (1)

Datasheet / COA / SDS

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Key Product Details

Source:	CHO
Accession #:	P01137
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human TGF-beta 1 GMP Protein, CF

Product Specifications

Source	Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein Ala279-Ser390 Manufactured and tested under cGMP guidelines.
Purity	>97%, by SDS-PAGE with silver staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala-Leu-Asp-Thr-Asn-Tyr-(Cys)-Phe-Ser-Ser
Predicted Molecular Mass	12.8 kDa (monomer)
SDS-PAGE	12 kDa, reducing conditions 24 kDa, non-reducing conditions
Activity	Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED₅₀ for this effect is 0.04-0.2 ng/mL. The specific activity of recombinant human TGF-beta 1 GMP is >1.0 x 10⁷ units/mg, which is calibrated against the human TGF‑ beta 1 Standard (NIBSC code: 89/514).

Recombinant Human TGF-beta 1 GMP Protein, CF Scientific Data Examples

	GMP-grade Recombinant Human TGF-beta 1 (Catalog # 240-GMP) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line. The ED₅₀for this effect is 0.04-0.2 ng/mL. Four independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP TGF-beta 1.
	1 μg/lane of GMP‑grade Recombinant Human TGF-beta 1 (Catalog # 240-GMP) was resolved with SDS-PAGE under reducing (R) and nonreducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

240-GMP

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 1

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non‑redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta RIII) and endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

Item	Value
References	Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982. Wahl, S.M. (2006) Immunol. Rev. 213:213. Chang, H. et al. (2002) Endocr. Rev. 23:787. Lin, J.S. et al. (2006) Reproduction 132:179. Hinck, A.P. et al. (1996) Biochemistry 35:8517. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261. Derynck, R. et al. (1985) Nature 316:701. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name	Transforming Growth Factor beta 1
Entrez Gene IDs	7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names	CEDLAP, DPD1, TGF beta, TGF beta1, TGF-beta 1, TGF-beta 1 protein, TGF-beta-1, TGFB, TGFB1, TGFbeta, TGFbeta 1, latency-associated peptide, transforming growth factor beta-1, transforming growth factor, beta 1

Citations (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human TGF-beta 1 GMP Protein, CF include:

Species: Rabbit
Sample Types: Whole Cells
Applications: Bioassay

K Yamashita et al. (2018), A Rabbit Corneal Endothelial Dysfunction Model Using Endothelial-Mesenchymal Transformed Cells Sci Rep, 8(1):16868.
PMID: 30442918

Recombinant Human TGF-beta 1 GMP Protein, CFGMP

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Key Product Details

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Product Specifications

Recombinant Human TGF-beta 1 GMP Protein, CF Scientific Data Examples

Formulation, Preparation and Storage

Carrier Free

Carrier Free

Reconstitution Calculator

Background: TGF-beta 1

Citations (1)

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