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Recombinant Human ST3GAL6 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10591-GT

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10591-GT-020

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ST3GAL6 protein
Asn40-Asp331, with N-termininal Fc

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

60.1 kDa

SDS-PAGE

67-81 kDa, under reducing conditions.

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to N-Acetyllactosamine.
The specific activity is >50 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human ST3GAL6 Fc Chimera Protein, CF

Recombinant Human ST3GAL6 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human ST3GAL6 Fc Chimera Protein (Catalog # 10591-GT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-81 kDa.

Formulation, Preparation and Storage

10591-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ST3GAL6

Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). ST3GAL6 is an alpha2,3-sialyltransferase and forms alpha2-3 linked sialic acid on Gal-beta 1,4-GlcNAc structure on glycoproteins and glycolipids (2). This enzyme has high specificity for some gangliosides and glycoproteins and may contribute to the formation of sialyl Lewis x (sLex), a carbohydrate important for cell-to-cell recognition and a blood group antigen (3). Study on various alpha2,3-sialyltransferases indicate that ST3GAL6 selectively sialylates EGFR therefore affects ERK and AKT signal transduction pathways and positively correlates to cell proliferation and colony formation, which is in sharp contrast to ST3GAL4 that selectively sialylates beta1 integrin (4).

References

  1. Varki, A. (1999) Glycobiology 2:25.
  2. Okajima, T. et al. (1999) J Biol Chem. 274:11479.
  3. Glavey, S. et al. (2014) Blood 124:1765.
  4. Qi, F. et al. (2020) FASEB J. 34:881.

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 6

Alternate Names

Sialyltransferase 10, SIAT10, ST3GALVI

Entrez Gene IDs

10402 (Human); 54613 (Mouse); 304023 (Rat)

Gene Symbol

ST3GAL6

UniProt

Additional ST3GAL6 Products

Product Documents for Recombinant Human ST3GAL6 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ST3GAL6 Fc Chimera Protein, CF

For research use only

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