Chinese Hamster Ovary cell line, CHO-derived human ST3GAL6 protein Asn40-Asp331, with N-termininal Fc
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
67-81 kDa, under reducing conditions
Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to N-Acetyllactosamine. The specific activity is >50 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). ST3GAL6 is an alpha 2,3-sialyltransferase and forms alpha 2-3 linked sialic acid on Gal-beta 1,4-GlcNAc structure on glycoproteins and glycolipids (2). This enzyme has high specificity for some gangliosides and glycoproteins and may contribute to the formation of sialyl Lewis x (sLex), a carbohydrate important for cell-to-cell recognition and a blood group antigen (3). Study on various alpha 2,3-sialyltransferases indicate that ST3GAL6 selectively sialylates EGFR therefore affects ERK and AKT signal transduction pathways and positively correlates to cell proliferation and colony formation, which is in sharp contrast to ST3GAL4 that selectively sialylates beta 1 integrin (4).
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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