Recombinant Human ST3GAL6 Fc Chimera Protein, CF

Name: Recombinant Human ST3GAL6 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10591-GT
Price: 581 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10591-GT

Citations (1)

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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source:	CHO
Accession #:	Q9Y274.1
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human ST3GAL6 Fc Chimera Protein, CF

Product Specifications for Recombinant Human ST3GAL6 Fc Chimera Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human ST3GAL6 protein Asn40-Asp331, with N-termininal Fc
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass	60.1 kDa
SDS-PAGE	67-81 kDa, under reducing conditions.
Activity	Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to N-Acetyllactosamine. The specific activity is >50 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human ST3GAL6 Fc Chimera Protein, CF

	Recombinant Human ST3GAL6 Fc Chimera Protein SDS-PAGE. 2 μg/lane of Recombinant Human ST3GAL6 Fc Chimera Protein (Catalog # 10591-GT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-81 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10591-GT

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: ST3GAL6

Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). ST3GAL6 is an alpha 2,3-sialyltransferase and forms alpha 2-3 linked sialic acid on Gal-beta 1,4-GlcNAc structure on glycoproteins and glycolipids (2). This enzyme has high specificity for some gangliosides and glycoproteins and may contribute to the formation of sialyl Lewis x (sLex), a carbohydrate important for cell-to-cell recognition and a blood group antigen (3). Study on various alpha 2,3-sialyltransferases indicate that ST3GAL6 selectively sialylates EGFR therefore affects ERK and AKT signal transduction pathways and positively correlates to cell proliferation and colony formation, which is in sharp contrast to ST3GAL4 that selectively sialylates beta 1 integrin (4).

Item	Value
References	Varki, A. (1999) Glycobiology 2:25. Okajima, T. et al. (1999) J Biol Chem. 274:11479. Glavey, S. et al. (2014) Blood 124:1765. Qi, F. et al. (2020) FASEB J. 34:881.
Long Name	ST3 beta-Galactoside alpha-2,3-Sialyltransferase 6
Entrez Gene IDs	10402 (Human); 54613 (Mouse); 304023 (Rat)
Alternate Names	CMP-NeuAc:beta-galactoside alpha-2,3-sialyltransferase VI, EC 2.4.99, EC 2.4.99.-, EC 2.4.99.9, SIAT10, ST3 beta-galactoside alpha-2,3-sialyltransferase 6, ST3GAL6, ST3GALVI, ST3GALVIsialyltransferase 10 (alpha-2,3-sialyltransferase VI), ST3Gal VI, Sialyltransferase 10, alpha2,3-sialyltransferase, type 2 lactosamine alpha-2,3-sialyltransferase

Citations for Recombinant Human ST3GAL6 Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human ST3GAL6 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

ZL Wu et al. (2021-01-01), Fluorescent glycan fingerprinting of SARS2 spike proteins Scientific Reports, 11(1):20428.
PMID: 34650101

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