Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein, CF

Name: Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI11066
Price: 548 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AVI11066

Product Datasheet / COA / SDS

Biotinylated

Catalog #

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Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	Q01638.4
Structure / Form:	Covalent homo-dimer Biotinylated via Avi-tag
Applications:	Bioactivity

View Full Technical Details for Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein, CF

Product Specifications for Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human ST2/IL-33R protein

Human ST-2 (Lys19-Ser328) Accession # Q01638.4	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Lys19

Predicted Molecular Mass

63 kDa

SDS-PAGE

90-105 kDa, under reducing conditions.

Activity

When Recombinant Human IL-33 (Catalog # 3625-IL) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human ST2/IL-33R Fc Chimera Avi-tag (Catalog # AVI11066) binds with an ED₅₀ of 90.0-720 ng/mL.

Scientific Data Examples for Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein, CF

	Biotinylated Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein Binding Activity. When Recombinant Human IL-33 (3625-IL) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein (Catalog # AVI11066) binds with an ED₅₀ of 90.0-720 ng/mL.
	Biotinylated Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein SDS-PAGE. 2 μg/lane of Biotinylated Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein (Catalog # AVI11066) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-105 kDa and 180-210 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11066

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ST2/IL-33R

Serum stimulation-2 (ST2), also known as Interleukin receptor like-1 (IL1RL1) and T1, is a member of the Interleukin-1 receptor superfamily family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of an extracellular domain (ECD) with three Ig-like domains, a transmembrane segment, and a cytoplasmic domain with an intracellular Toll/interleukin-1 receptor (TIR) domain (3, 4). Within the ECD, human ST2 shares 68% and 64% amino acid sequence identity with mouse and rat ST2, respectively. Alternate splicing of human ST2 generates a soluble isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5-8). It binds IL33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL1R AcP, a shared signaling subunit that also associates with IL1RI and IL1R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL1RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12-14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL33 signaling by full-length ST2 (10, 13‑15). Our Avi-tag Biotinylated ST2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Item	Value
References	Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31. Tominaga, S. et al. (1992) Biochim. Biophys. Acta. 1171:215. Li, H. et al. (2000) Genomics 67:284. Schmitz, J. et al. (2005) Immunity 23:479. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979. Brint, E.K. et al. (2004) Nat. Immunol. 5:373. Weinberg, E.O. et al. (2002) Circulation 106:2961. Sanada S. et al. (2007) J. Clin. Invest. 117:1538. Palmer, G. et al. (2008) Cytokine 42:358. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051. Verri, Jr. W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723. Miller, A.M. et al. (2008) J. Exp. Med. 205:339. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
Long Name	Interleukin 33 Receptor
Entrez Gene IDs	9173 (Human); 17082 (Mouse)
Alternate Names	DER4, DER4ST2growth stimulation-expressed, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, MGC32623, Protein ST2, ST2, ST2L, ST2V, T1, T1homolog of mouse growth stimulation-expressed, interleukin 1 receptor-like 1, interleukin 1 receptor-related protein, interleukin-1 receptor-like 1

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Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein, CF

Scientific Data Examples for Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: ST2/IL-33R

Citations for Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein, CF

Product Documents for Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein, CF

Product Datasheets

COA

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