Recombinant Human IL-33 Protein

Name: Recombinant Human IL-33 Protein
Brand: R&D Systems
SKU: 3625-IL
Rating: 5 (6 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3625-IL

Citations (49)

(6)

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Key Product Details

Source:	E. coli
Accession #:	O95760.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-33 Protein

Product Specifications for Recombinant Human IL-33 Protein

Source	E. coli-derived human IL-33 protein Ser112-Thr270
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser112
Predicted Molecular Mass	18-21 kDa
Activity	Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 0.06-0.24 ng/mL. Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Recombinant Human IL-33 Protein

	Recombinant Human IL-33 Protein Bioactivity Recombinant Human IL-33 (Catalog # 3625‑IL) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED₅₀ for this effect is 0.06-0.24 ng/mL.
	Recombinant Human IL-33 Protein SDS-PAGE 1 μg/lane of Recombinant Human IL-33 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3625-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3625-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF‑HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL‑33 was identified based on sequence and structural homology with IL‑1 family cytokines (3). DVS 27, NF‑HEV, and IL‑33 share 100% amino acid sequence identity. IL‑33 is constitutively expressed in smooth muscle and airway epithelia. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL‑1 alpha or IL‑1 beta stimulation (1, 3). Similar to IL‑1, IL‑33 can be cleaved in vitro by caspase‑1, generating an N‑terminal fragment that is slightly shorter than the C‑terminal fragment (3, 4). The N‑terminal portion of full length IL‑33 contains a predicted bipartite nuclear localization sequence and a homeodomain‑like helix‑turn‑helix DNA binding domain. By immunofluorescence, full length IL‑33 localizes to the nucleus in HUVECs and transfectants (2). The C‑terminal fragment, corresponding to mature IL‑33, binds and triggers signaling through mast cell IL‑1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5‑7). A ternary signaling complex is formed by the subsequent association of IL‑33 and ST2L with IL‑1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL‑33 in vitro induces IL‑5 and IL‑13 secretion (3). In vivo administration of mature IL‑33 promotes increased production of IL‑5, IL‑13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature human IL‑33 share
52‑58% aa sequence identity with mouse and rat IL‑33. Human IL‑33 shares less than 20% aa sequence identity with other IL‑1 family proteins.

Item	Value
References	Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69. Schmitz, J. et al. (2005) Immunity 23:479. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323. Xu, D. et al. (1998) J. Exp. Med. 187:787. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. 95:6930. Dinarello, C.A. (2005) Immunity 23:461. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Long Name	Interleukin 33
Entrez Gene IDs	90865 (Human); 77125 (Mouse)
Alternate Names	C9orf26, C9orf26chromosome 9 open reading frame 26 (NF-HEV), DKFZp586H0523, DVS27, DVS27-related protein, IL-1F11, IL-33, IL1F11, IL33, Interleukin-1 family member 11, NF-HEV, NF-HEVNFEHEV, NFHEV, Nuclear factor from high endothelial venules, RP11-575C20.2, interleukin 33, interleukin-33

Citations for Recombinant Human IL-33 Protein (49)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-33 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Wang et al. (2023-01-01), Changes in type 2 innate lymphoid cells and serum cytokines in sublingual immunotherapy in pediatric patients with allergic rhinitis BMC pediatrics, 23(1):13.
PMID: 36624390
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

DAB Rex et al. (2022-01-01), Temporal Quantitative Phosphoproteomics Profiling of Interleukin-33 Signaling Network Reveals Unique Modulators of Monocyte Activation Cells, 11(1).
PMID: 35011700
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Rasheed et al. (2022-01-01), The Merkel Cell Polyomavirus T-Antigens and IL-33/ST2-IL1RAcP Axis: Possible Role in Merkel Cell Carcinoma International Journal of Molecular Sciences, 23(7).
PMID: 35409061
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Akoto et al. (2022-01-01), IL-33 Induces an Antiviral Signature in Mast Cells but Enhances Their Permissiveness for Human Rhinovirus Infection Viruses, 14(11).
PMID: 36366528
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Zheng et al. (2021-01-01), Exogenous oxidative stress suppresses IL-33 -driven proliferation programming in group 2 innate lymphoid cells International immunopharmacology, 95(0):107541.
PMID: 33756232
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Cristinzia et al. (2021-01-01), IL-33 and Superantigenic Activation of Human Lung Mast Cells Induce the Release of Angiogenic and Lymphangiogenic Factors Cells, 10(1).
PMID: 33445787
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

HH Park et al. (2020-01-01), TGF-&beta secreted by human umbilical cord blood-derived mesenchymal stem cells ameliorates atopic dermatitis by inhibiting secretion of TNF-&alpha and IgE Stem Cells, 0(0).
PMID: 32277785
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Y Imoto et al. (2020-01-01), Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis Biomolecules, 10(11).
PMID: 33218117
Species: Human
Sample Types: Cells
Applications: Cell Culture

T Petrova et al. (2020-01-01), p38 MAPK signalling regulates cytokine production in IL-33 stimulated Type 2 Innate Lymphoid cells Sci Rep, 10(1):3479.
PMID: 32103032
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Hochdörfer et al. (2019-01-01), Expression of c-Kit discriminates between two functionally distinct subsets of human type 2 innate lymphoid cells Eur. J. Immunol., 0(0).
PMID: 30892687
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Qin et al. (2019-01-01), The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis Cytokine, 117(0):84-90.
PMID: 30831444
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

JH Bernink et al. (2019-01-01), c-Kit-positive ILC2s exhibit an ILC3-like signature that may contribute to IL-17-mediated pathologies Nat. Immunol., 0(0).
PMID: 31263279
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

S Sugihara et al. (2019-01-01), TNF-Î± and IL-17A induce the expression of lympho-epithelial Kazal-type inhibitor in epidermal keratinocytes J. Dermatol. Sci., 0(0).
PMID: 31501002
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Mishima et al. (2019-01-01), Interleukin-33 delays recovery of mucosal inflammation via downregulation of homeostatic ABCG5/8 in the colon Lab. Invest., 0(0).
PMID: 31641224
Species: Human
Sample Types: cell culture
Applications: Whole Cells

JF Zhang et al. (2019-01-01), IL-33/ST2 axis promotes glioblastoma cell invasion by accumulating tenascin-C Sci Rep, 9(1):20276.
PMID: 31889095
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Liu et al. (2018-01-01), IL-33 Initiates Vascular Remodelling in Hypoxic Pulmonary Hypertension by up-Regulating HIF-1? and VEGF Expression in Vascular Endothelial Cells EBioMedicine, 0(0).
PMID: 29921553
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Q Miao et al. (2018-01-01), Seasonal variation in circulating group 2 innate lymphoid cells in mugwort-allergic asthmatics during and outside pollen season Allergy Asthma Clin Immunol, 14(0):6.
PMID: 29449864
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Matsumoto et al. (2018-01-01), Soluble ST2 suppresses IL-5 production by human basophilic KU812 cells, induced by epithelial cell-derived IL-33 Allergol Int, 0(0).
PMID: 29941231
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

WI Ryu et al. (2018-01-01), IL-33 down-regulates CLDN1 expression through the ERK/STAT3 pathway in keratinocytes J. Dermatol. Sci., 0(0).
PMID: 29534857
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

XH Wang et al. (2017-01-01), IL?33 restricts invasion and adhesion of trophoblast cell line JEG3 by downregulation of integrin ?4?1 and CD62L Mol Med Rep, 0(0).
PMID: 28765940
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Wang et al. (2017-01-01), IL-33 blockade suppresses tumor growth of human lung cancer through direct and indirect pathways in a preclinical model Oncotarget, 8(40):68571-68582.
PMID: 28978138
Species: Human
Sample Types: Whole Cells

A Taracanova et al. (2017-01-01), SP and IL-33 together markedly enhance TNF synthesis and secretion from human mast cells mediated by the interaction of their receptors Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 28461492
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JF Zhang et al. (2017-01-01), IL?33 enhances glioma cell migration and invasion by upregulation of MMP2 and MMP9 via the ST2-NF-?B pathway Oncol. Rep., 38(4):2033-2042.
PMID: 28849217
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Fang et al. (2017-01-01), IL-33 promotes colon cancer cell stemness via JNK activation and macrophage recruitment Cancer Res, 0(0).
PMID: 28249897
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay

D Díaz-Jimén et al. (2017-01-01), A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis Sci Rep, 7(1):10180.
PMID: 28860510
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

V Lombardi et al. (2017-01-01), Effector and regulatory dendritic cells display distinct patterns of miRNA expression Immun Inflamm Dis, 0(0).
PMID: 28497578
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JE Miller et al. (2017-01-01), Interleukin-33 modulates inflammation in endometriosis Sci Rep, 7(1):17903.
PMID: 29263351
Species: Human
Sample Types: Whole Cells

S Lopez-Last et al. (2017-01-01), A functional DC cross talk promotes human ILC homeostasis in humanized mice Blood Adv, 1(10):601-614.
PMID: 29296702
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation Sci Rep, 6(0):25171.
PMID: 27142573
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), Soluble IL-33 receptor sST2 inhibits colorectal cancer malignant growth by modifying the tumour microenvironment Nat Commun, 7(0):13589.
PMID: 27882929
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells Sci Rep, 6(0):36795.
PMID: 27827428
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), Functional IL-33 receptors are expressed in early progenitor stages of allergy-related granulocytes Immunology, 0(0).
PMID: 27568595
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SM Bal et al. (2016-01-01), IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs Nat Immunol, 0(0).
PMID: 27111145
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Maywald R et al. (2015-01-01), IL-33 activates tumor stroma to promote intestinal polyposis. Proc Natl Acad Sci U S A, 112(19):E2487-96.
PMID: 25918379
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Bu X et al. (2015-01-01), IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production. J Transl Med, 13(0):381.
PMID: 26675669
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Kim J et al. (2015-01-01), Interleukin-33/ST2 axis promotes epithelial cell transformation and breast tumorigenesis via upregulation of COT activity. Oncogene, 34(38):4928-38.
PMID: 25531326
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kempuraj D et al. (2015-01-01), Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins alpha-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators. PLoS ONE, 10(8):e0135776.
PMID: 26275153
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Levescot A et al. (2014-01-01), BCR-ABL-induced deregulation of the IL-33/ST2 pathway in CD34+ progenitors from chronic myeloid leukemia patients. Cancer Res, 74(10):2669-76.
PMID: 24675360
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Teunissen M et al. (2014-01-01), Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients. J Invest Dermatol, 134(9):2351-60.
PMID: 24658504
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wong et al. (2014-01-01), Eosinophils regulate peripheral B cell numbers in both mice and humans. J Immunol, 192(8):3548-58.
PMID: 24616476
Stojkovic S et al. (2014-01-01), Interleukin-33 induces urokinase in human endothelial cells--possible impact on angiogenesis. J Thromb Haemost, 12(6):948-57.
PMID: 24702774
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Thierry A et al. (2014-01-01), The alarmin concept applied to human renal transplantation: evidence for a differential implication of HMGB1 and IL-33. PLoS ONE, 9(2):e88742.
PMID: 24586382
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Qiu H et al. (2013-01-01), Muramyl dipeptide mediated activation of human bronchial epithelial cells interacting with basophils: a novel mechanism of airway inflammation. Clin Exp Immunol, 172(1):81-94.
PMID: 23480188
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Liu K et al. (2013-01-01), Anti-inflammatory and anti-allergic activities of Pentaherb formula, Moutan Cortex (Danpi) and gallic acid. Molecules, 18(3):2483-500.
PMID: 23439564
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2012-01-01), Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33. J. Exp. Med., 209(8):1505-17.
PMID: 22802353
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Vasiadi M et al. (2012-01-01), Serum neurotensin (NT) is increased in psoriasis and NT induces vascular endothelial growth factor release from human mast cells. Br. J. Dermatol., 166(6):1349-52.
PMID: 22283733
Species: Human
Sample Types: Whole Blood
Applications: Bioassay

Sahlander K et al. (2012-01-01), Daily exposure to dust alters innate immunity. PLoS ONE, 7(2):e31646.
PMID: 22355383
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cherry WB et al. (2008-01-01), A novel IL-1 family cytokine, IL-33, potently activates human eosinophils. J. Allergy Clin. Immunol., 121(6):1484-90.
PMID: 18539196
Hong YS et al. (), Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis. J Korean Med Sc26(9):1132-9.
PMID: 21935266

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Customer Reviews for Recombinant Human IL-33 Protein (6)

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Recombinant Human IL-33 Protein

Name: Anonymous

Application: CellProlif

Reason for Rating: Human SMCs were tested for proliferation in response to IL-33.

Verified Customer | Posted 12/05/2021
Recombinant Human IL-33 Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 08/12/2021
Recombinant Human IL-33 Protein

Name: Anonymous

Application: ELISA standard

Verified Customer | Posted 06/09/2020

We used this protein in an in-house ELISA along with mAb (3625) and pAb (AF3625) to quantify IL-33 in human serum and plasma. This combination could not detect IL-33 in our samples but generated a good standard curve.
Recombinant Human IL-33 Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 09/16/2019
Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 03/29/2018
Recombinant Human IL-33 Protein

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 11/09/2017

AF3625 was used as both the capture and the detection antibody for the sandwich ELISA for IL-33. The immunoassay standard was 3625-IL. Assay had sensitivity of ~1pg/ml. Many healthy human samples were not detectable.

Recombinant Human IL-33 Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human IL-33 Protein

Scientific Data Examples for Recombinant Human IL-33 Protein

Formulation, Preparation and Storage

Carrier Free

3625-IL

Carrier

3625-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-33

Citations for Recombinant Human IL-33 Protein (49)

Customer Reviews for Recombinant Human IL-33 Protein (6)

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Recombinant Human IL-33 Protein

Recombinant Human IL-33 Protein

Recombinant Human IL-33 Protein

Recombinant Human IL-33 Protein

Recombinant Human IL-33 Protein

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