Recombinant Human SREC-I/SCARF1 Fc Chimera Protein, CF

Name: Recombinant Human SREC-I/SCARF1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 2409-SR
Price: 460 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 2409-SR

Citations (5)

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Key Product Details

Source:	NS0
Accession #:	Q14162
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

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Product Specifications for Recombinant Human SREC-I/SCARF1 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human SREC-I/SCARF1 protein

Human SREC-I (Ser20-Thr421) Accession # Q14162	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser20

Predicted Molecular Mass

69.1 kDa (monomer)

SDS-PAGE

100 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant human AcLDL at 2 µg/mL (100 µL/well) can bind Recombinant Human SREC‑I/SCARF1 Fc Chimera with a linear range of 0.3-25 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2409-SR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SREC-I/SCARF1

The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share a common ability to bind and internalize modified forms of low density lipoproteins (LDL) such as acetylated LDL (AcLDL) and oxidized LDL(OxLDL) (1 - 3). Family members are classified alphabetically. They play important roles in lipid metabolism, in host defence and in the regulation of acquired immunity (2, 4). Scavenger receptor expressed by endothelial cells-I (SREC-I) and SREC-II are two proteins that belong to the F type scavenger receptor group (SR-F1 and SR-F2). The full length cDNA of human SREC-I encodes an 830 amino acid (aa) type I transmembrane protein which contains a 19 aa signal peptide, a 402 aa extracellular region, a 21 aa transmembrane segment, and a 388 aa long cytoplasmic domain. The extracellular region contains ten EGF-like repeats (five of which fit the exact consensus sequence for an EGF-like domain) while the cytoplasmic domain is rich in serine and proline in the N-terminal half, and glycine in the C-terminal segment (5, 6). In addition to the full length form, four SREC-I isoforms exist. Two show insertions of a stop codon in EGF-like domain #8, resulting in mature soluble forms of 323 aa and 318 aa, respectively. A third isoform deletes part of domain #8 plus domains #9 and #10; it continues in-frame to generate a mature transmembrane protein of 725 aa. The last isoform shows only cytoplasmic splicing, with 72 aa substituted for the last 332 aa of the full length form. All three transmembrane forms bind acetylated LDL (6). Native SREC-I is approximately 150 kDa in size, and expressed by endothelial cells, macrophages and fetal neurons (7, 8). In the extracellular region human SREC-I is 76% aa identical to mouse SREC-I. The extracellular regions of human SREC-I and II are 53% aa identical.

Item	Value
References	Horiuchi, S. et al. (2003) Amino Acids 25:283. Greaves, D.R. and S. Gordon (2005) J. Lipid Res. 46:11. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649. Adachi, H. et al. (1997) J. Biol. Chem. 272:31217. Adachi, H. and M. Tsujimoto (2002) J. Biol. Chem. 277:24014. Shibata, M. et al. (2004) J. Biol. Chem. 279:40084. Tanura, Y. et al. (2004) J. Biol. Chem. 279:30938.
Long Name	Scavenger Receptor Expressed by Endothelial Cells 1
Entrez Gene IDs	8578 (Human)
Alternate Names	Acetyl LDL receptor, KIAA0149endothelial cells scavenger receptor, MGC47738, SCARF1, SR-F1, SREC-I, SREC1, SRECI, SRECscavenger receptor expressed by endothelial cells, Scavenger receptor expressed by endothelial cells 1, scavenger receptor class F, member 1

Citations for Recombinant Human SREC-I/SCARF1 Fc Chimera Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human SREC-I/SCARF1 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

DA Patten et al. (2017-01-01), SCARF-1 promotes adhesion of CD4+ T cells to human hepatic sinusoidal endothelium under conditions of shear stress Sci Rep, 7(1):17600.
PMID: 29242513
Species: Mouse
Sample Types: Protein
Applications: Binding Assay

Biedron R et al. (2015-01-01), Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages. PLoS ONE, 10(4):e0123293.
PMID: 25849867
Baur S et al. (2014-01-01), A nasal epithelial receptor for Staphylococcus aureus WTA governs adhesion to epithelial cells and modulates nasal colonization. PLoS Pathog, 10(5):e1004089.
PMID: 24788600
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay

Narazaki M et al. (2010-01-01), Oligo-guanosine nucleotide induces neuropilin-1 internalization in endothelial cells and inhibits angiogenesis. Blood, 116(16):3099-107.
PMID: 20606164
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay

Narazaki M et al. (2008-01-01), Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A. Blood, 111(8):4126-36.
PMID: 18272814

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Recombinant Human SREC-I/SCARF1 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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