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Recombinant Human Soggy-1/DkkL1 Protein

Bio-Techne includes R&D Systems | Catalog # 1549-S1

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Carrier Free
1549-S1-025/CF

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With Carrier
1549-S1-025

Key Product Details

Source

NS0

Accession #

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Soggy-1/DkkL1 protein
Ala31-Leu242, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala31

Predicted Molecular Mass

25.3 kDa

SDS-PAGE

40 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells.
The ED50 for this effect is 2-6 µg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1549-S1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1549-S1/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Soggy-1/DkkL1

Soggy-1, also known as DkkL1 (Dickkopf-like 1), is a secreted protein that is related to the Dickkopf family of Wnt antagonists. Dkk-1, -2, -3, and -4 each have two cysteine-rich domains separated by a linker. Soggy-1 does not contain cysteine-rich domains but bears some sequence homology with the N-terminal regions of Dkk proteins (1, 2). The human Soggy-1 cDNA encodes a 242 amino acid (aa) precursor that includes a 30 aa signal sequence (2). Human Soggy-1 shares approximately 65% aa sequence identity with mouse and rat Soggy-1 and 15 - 20% aa sequence identity with human Dkk-1, -2, -3, and -4. Mouse Soggy-1 is expressed at various sites in the embryo but in the adult is primarily found in the testes (3 - 5). Soggy-1 transcription is regulated by the spermatocyte specific factor, RFX2 (6). The regulatory elements for Soggy-1 lie very close to those of TEAD-2, a transcription factor that is expressed very early in development (4). Soggy-1 and TEAD-2 are co-expressed in preimplantation embryos and embryonic stem cells, but differentiated cells express only one or the other (4, 5). During development, Soggy-1 is first detectable at the onset of sexual differentiation (4). Soggy-1 is localized to the acrosome in developing spermatocytes and mature spermatozoa (3, 4). Soggy-1, as expressed in developing mouse spermatocytes, is a 34 kDa N-glycosylated protein. This glycosylation is not present on Soggy-1 in mature spermatozoa, although the apparent molecular weight suggests the presence of some post-translational modification (3). Two shorter forms of Soggy-1 have been described that result from the use of internal methionine residues for initiation (4).

References

  1. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
  2. Krupnik, V.E. et al. (1999) Gene 238:301.
  3. Kohn, M.J. et al. (2005) Mol. Reprod. Dev. 71:516.
  4. Kaneko, K.J. and M.L. DePamphilis (2000) Nucleic Acids Res. 28:3982.
  5. Kaneko, K.J. et al. (2004) Mol. Cell. Biol. 24:1968.
  6. Horvath, G.C. et al. (2004) Biol. Reprod. 71:1551.

Long Name

Dickkopf-like Protein 1

Alternate Names

DkkL1, SGY1, Soggy1

Entrez Gene IDs

27120 (Human); 50722 (Mouse)

Gene Symbol

DKKL1

UniProt

Additional Soggy-1/DkkL1 Products

Product Documents for Recombinant Human Soggy-1/DkkL1 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Soggy-1/DkkL1 Protein

For research use only

Citations for Recombinant Human Soggy-1/DkkL1 Protein (3)

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