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Recombinant Human SLURP2 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10035-SP

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10035-SP-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human SLURP2 protein
MD Human IgG1
(Pro100-Lys330)
IEGR Human SLURP2
(Ile23-Asp97)
Accession # P0DP57
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

35 kDa

SDS-PAGE

36-43 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Nicotinic Acetylcholine R alpha 7/CHRNA7 (Novus Catalog # H00001139) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SLURP2 Fc Chimera binds with an ED50 of 1-6 μg/mL.

Scientific Data Images for Recombinant Human SLURP2 Fc Chimera Protein, CF

Recombinant Human SLURP2 Fc Chimera Protein Binding Activity

Recombinant Human SLURP2 Fc Chimera Protein Binding Activity

When Recombinant Human Nicotinic Acetylcholine R alpha 7/CHRNA7 (Novus Catalog # H00001139) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SLURP2 Fc Chimera (Catalog # 10035-SP) binds with an ED50 of 1-6 μg/mL.
Recombinant Human SLURP2 Fc Chimera Protein SDS-PAGE

Recombinant Human SLURP2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human SLURP2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 36-43 kDa and 70-85 kDa, respectively.

Formulation, Preparation and Storage

10035-SP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: SLURP2

Secreted Ly-6/uPAR domain-containing protein 2 (SLURP2) is expressed primarily in epithelial and immune cells and regulates growth and differentiation of epithelial cells (1, 2). It is part of the Ly6/neurotoxin superfamily and contains the ‘three-finger' UPAR/Ly6 domain, which has a beta-structural core and three protruding loops (2, 3). Mature SLURP2 is a secreted protein which is comprised of 75 amino acids. Human SLURP2 shares 65% and 25% aa identity with mouse and rat SLURP2, respectively. SLURP2 is demonstrated to lessen the tumorigenic activity of nitrosamines on Het-1A cells (4), reduce inflammation on human intestinal epithelial cells and Immunocytes such as CEM and U937 (5). SLURP2 can interact with alpha3, alpha4, alpha5, alpha7, beta2, beta4, and possibly alpha6 nicotinic acetylcholine receptors (nAChRs) as well as M1 and M3 muscarinic acetylcholine receptors (3, 6). In addition, SLURP2 controls the proliferation of keratinocytes and epithelial cancer cells via nAChRs on the cell surface (1, 6, 7). It has been suggested SLURP2 may be involved in the pathophysiology of psoriasis through its role in keratinocyte hyper-proliferation and/or T cell differentiation/activation (8).

References

  1. Arredondo, J. et al. (2006) J. Cell. Physiol. 208:238.
  2. Moriwaki, Y. et al. (2015) Int. Immunopharmacol. 29:71.
  3. Lyukmanova, E.N. et al. (2016) Sci. Rep. 6:30698.
  4. Arredondo, J. et al. (2007) Life. Sci. 80:2243.
  5. Chernyavsky, AI. et al. (2014) Biomed. Res. Int. 2014:609086.
  6. Lyukmanova, E.N. et al. (2014) Acta. Naturae. 6:60.
  7. Lyukmanova, E.N. et al. (2018) Br. J. Pharmacol. 175:1973.
  8. Tsuji, H. et al. (2003) Genomics 81:26.

Long Name

Secreted Ly-6/uPAR-related Protein 2

Alternate Names

SLURP-2

Entrez Gene IDs

432355 (Human)

Gene Symbol

SLURP2

UniProt

Additional SLURP2 Products

Product Documents for Recombinant Human SLURP2 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human SLURP2 Fc Chimera Protein, CF

For research use only

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