Product Specifications for Recombinant Human SLITRK1 Protein, CF
Mouse myeloma cell line, NS0-derived human SLITRK1 protein Ala16-Ser616, with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
100-115 kDa, reducing conditions
Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 100 ng on a nitrocellulose-coated microplate.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
SLITRK1 (Slit and Trk-like family member 1) is a charter member of the SLITRK family of proteins (1, 2). This family includes six members, all of which contain a slit-like extracellular region, and all of which (save for SLITRK1) contain a Trk-like cytoplasmic region (3). Human SLITRK1 is a type I transmembrane protein 696 amino acids (aa) in length. It contains a 17 aa signal sequence, a 605 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 53 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in slit. Each LRD (aa 57 - 200; aa 374 - 517) contains six leucine-rich repeats (LRRs), followed by a Cys-rich, C-terminal LRR (aa 212 - 262; aa 529 - 579). The exact function of the LRRs is not known. Based on other LRRs, it might be assumed that they mediate protein-protein-interaction (4). There are multiple, single aa mutations in the ECD. Their significance is unclear (5). One truncating frameshift mutation is known that shows a unique 27 aa substitution after Arg 421 (6). This 448 aa form is associated with Tourette’s Syndrome. As noted, the cytoplasmic region is unique among SLITRK family members. It contains no known signaling motifs, which is in contrast to SLITRK2 - 6 which show TrkA-like tyrosine phosphorylation sites. This difference is reflected in their function. SLITRK1 is a protein that is found in adult neurons of the cerebrum, thalamus and hippocampus. It induces neurite outgrowth whereas SLITRK2 - 6 block neurite extension (3). Human SLITRK1 shares 99%, 98% and 99% aa sequence identity with canine, mouse and bovine SLITRK1, respectively.
Aruga, J. et al. (2003) Gene 315:87.
Nagase, T. et al. (2001) DNA Res. 8:179.
Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
Enkhbayar, P. et al. (2003) Proteins 54:394.
Zuchner, S. et al. (2006) Mol. Psychiat. 11:888.
Abelson, J.F. et al. (2005) Science 310:317.
SLIT and NTRK-like Family, Member 1
Entrez Gene IDs
114798 (Human); 76965 (Mouse)
FLJ54428, KIAA1910KIAA0918, LRRC12, Leucine-rich repeat-containing protein 12, RP11-395N17.1, SLIT and NTRK-like family, member 1, SLIT and NTRK-like protein 1, SLITRK1, TTM, leucine rich repeat containing 12, slit and trk like gene 1
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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