Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF

Name: Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1137-SL
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1137-SL

Citations (7)

(1)

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Key Product Details

Source:	NS0
Accession #:	AAA51948
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF

Product Specifications for Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human Siglec-3/CD33 protein

Human Siglec-3 (Asp18-His259) (Val257Leu) Accession # AAA51948	DIEGRMD	Human IgG₁(Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Asp18

Predicted Molecular Mass

53.4 kDa (monomer)

SDS-PAGE

67-85 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The ED₅₀ for this effect is 1‑4 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1137-SL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Siglec-3/CD33

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11 (1 - 3). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (1, 2). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasma tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

Human Siglec-3 is alternatively known as myeloid cell surface antigen CD33 and GP67. Human Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (1, 4). Siglec-3 expression is restricted to cells of myelomonocytic lineage (2). It binds sialic acid preferring alpha 2,3- linkage over alpha 2,6- linkage (5). Studies indicated that Siglec-3 recruits SHP-1 and SHP-2 to its ITIMs (6, 7). When co-crosslinking with Fc gamma R1, Siglec-3 inhibits tyrosine phosphorylation and calcium mobilization, suggesting Siglec-3 can mediate inhibitory signals (7).

Item	Value
References	Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337. Crocker, P.R. and A. Varki (2001) Immunology 103:137. Angata, T. et al. (2002) J. Biol. Chem. 277:24466. Simmons, D. and B. Seed (1988) J. Immunol. 141:2797. Freeman, S.D. et al. (1995) Blood 85:2002. Taylor, V.C. et al. (1999) J. Biol. Chem. 274:11505. Ulyanova, T. et al. (1999) Eur. J. Immunol. 29:3440.
Long Name	Sialic Acid Binding Ig-like Lectin 3
Entrez Gene IDs	945 (Human); 12489 (Mouse); 102117580 (Cynomolgus Monkey)
Alternate Names	CD33, CD33 antigen, CD33 antigen (gp67), CD33 molecule, FLJ00391, SIGLEC3gp67, Sialic acid-binding Ig-like lectin 3, Siglec-3, Siglec3, gp67, myeloid cell surface antigen CD33, p67, sialic acid binding Ig-like lectin 3

Citations for Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF (7)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Recombinant Proteins
Applications: Western Blot Control

OS Adeniji et al. (2021), Siglec-9 defines and restrains a natural killer subpopulation highly cytotoxic to HIV-infected cells PloS Pathogens, 17(11):e1010034.
PMID: 34762717
Species: Human
Sample Types: Natural Protein
Applications: Bioassay

J Terävä et al. (2019), Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma PLoS ONE, 14(7):e0219480.
PMID: 31344060
Species: N/A
Sample Types: Protein
Applications: Binding Assay

Ishida A et al. (2014), Negative regulation of Toll-like receptor-4 signaling through the binding of glycosylphosphatidylinositol-anchored glycoprotein, CD14, with the sialic acid-binding lectin, CD33. J Biol Chem, 289(36):25341-50.
PMID: 25059667
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay

Chen et al. (2014), Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1. Elife, 3(0):e04066.
PMID: 25187624
Species: Virus
Sample Types: Virus
Applications: Surface Plasmon Resonance

Zou Z et al. (2011), Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids. PLoS ONE, 6(9):e24559.
PMID: 21931755
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Robak LA et al. (2009), Molecular basis of the interactions of the Nogo-66 receptor and its homolog NgR2 with myelin-associated glycoprotein: development of NgROMNI-Fc, a novel antagonist of CNS myelin inhibition. J. Neurosci., 29(18):5768-83.
PMID: 19420245
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Hasegawa Y et al. (2004), Promotion of axon regeneration by myelin-associated glycoprotein and Nogo through divergent signals downstream of Gi/G. J. Neurosci., 24(30):6826-32.
PMID: 15282288

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Customer Reviews for Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF (1)

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Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF

Name: Jenna Nguyen

Application: Enzymatic activity in vitro

Verified Customer | Posted 06/17/2019

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Product Documents for Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF

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Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Siglec-3/CD33

Citations for Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF (7)

Customer Reviews for Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF (1)

Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF

Product Documents for Recombinant Human Siglec-3/CD33 Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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