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Recombinant Human Semaphorin 7A Fc Chimera Protein, CF

Catalog # 2068-S7 | R&D Systems, Inc. a Bio-Techne Brand
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2068-S7-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Semaphorin 7A protein
Human Semaphorin 7A
(His47-Ala648)
Accession # O75326
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His47

Predicted Molecular Mass

94.8 kDa (monomer)

SDS-PAGE

100-105 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Plexin C1 (Catalog # 3887-PC) is coated at 5 μg/mL, Recombinant Human Semaphorin 7A Fc Chimera binds with an apparent K<5 nM.

Formulation, Preparation and Storage

2068-S7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 7A

Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules (1 ‑ 4). On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen (4). Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen and thymus (1 ‑ 5). Human Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 604 aa extracellular domain (ECD) including Sema and C2‑type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature human Sema7A shares 89%, 89%, 88%, 86% and 90% aa identity with mouse, rat, canine, bovine and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif (4). Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta1 integrins such as alpha1 beta1 (6 ‑ 10). Sema7A signaling through the two receptors may cause opposing effects (8). Sema7A is an immune semaphorin, with expression and activity on CD4+CD8+ thymocytes, activated T cells, macrophages and microglia (2, 9 ‑ 12). T cell Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation (5, 6). However, on the T cells themselves, Sema7A downregulates TCR signaling by promoting TCR internalization, modulating T cell responses (9). In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta -induced lung fibrosis (12). Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract (8, 10, 13). 

References

  1. Yazdani, U. and J.R. Terman (2006) Genome Biol. 7:211.
  2. Kikutani, H. et al. (2007) Adv. Immunol. 93:121.
  3. Sato, Y. and Takahashi H. (1998) Biochim. Biophys. Acta. 1443:419.
  4. Yamada, A. et al. (1999) J. Immunol. 162:4094.
  5. Holmes, S. et al. (2002) Scand. J. Immunol. 56:270.
  6. Suzuki, K. et al. (2007) Nature 446:680.
  7. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
  8. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
  9. Czopik, A.K. et al. (2006) Immunity 24:591.
  10. Pasterkamp, R.J. et al. (2003) Nature 424:398.
  11. Mine, T. et al. (2000) Tissue Antigens 55:429.
  12. Kang, H.-R. et al. (2007) J. Exp. Med. 204:1083.
  13. Delorme, G. et al. (2005) Biol. Cell 97:589.

Alternate Names

CD108, H-Sema-L, Sema7A, SEMAL

Entrez Gene IDs

8482 (Human); 20361 (Mouse)

Gene Symbol

SEMA7A

UniProt

Product Documents for Recombinant Human Semaphorin 7A Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Semaphorin 7A Fc Chimera Protein, CF

For research use only

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