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Recombinant Human Semaphorin 4B Fc Chimera Protein, CF

Catalog # 10262-S4 | R&D Systems, Inc. a Bio-Techne Brand
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10262-S4-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Semaphorin 4B protein
Human Semaphorin 4B
(Leu44-Glu717)
Accession # Q9NPR2.4
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

101 kDa

SDS-PAGE

105-118 kDa, under reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human Semaphorin 4B Fc Chimera (Catalog # 10262-S4), immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Human Semaphorin 4B Fc Chimera Protein, CF

Recombinant Human Semaphorin 4B Fc Chimera Protein SDS-PAGE

Recombinant Human Semaphorin 4B Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human Semaphorin 4B Fc Chimera (Catalog # 10262-S4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-118 kDa and 210-240 kDa, respectively.

Formulation, Preparation and Storage

10262-S4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4B

Semaphorin 4B (Sema4B) is a 95-100 kDa, class IV member of the Semaphorin family of proteins (1). Mature human Sema4B is a type I transmembrane glycoprotein that is 794 amino acids (aa) in length. It contains a 674 aa extracellular domain (ECD) that is characterized by one Sema domain, a PSI region, and an Ig-like C2-type domain. Within the ECD, human Sema4B shares 85% and 86% aa identity with the mouse and rat Sema4B, respectively (1, 2). Sema4B is expressed in hippocampal neurons, glial cells, and immune cells (3, 4). It colocalizes and interacts with PSD-95 and participates in the formation or functioning of glutamatergic synapses (4). Sema4B-/- mice display reduced proliferation of astrocytes after CNS injury (5). In the immune system, Sema4B is a negative regulator of basophil-mediated immune response and is implicated in the development of lung cancer (6, 7).

References

  1. Gurrapu, S. et al. (2016) Cell Adh. Migr. 10:675.
  2. Alto, L. and J.R. Terman (2017) Methods. Mol. Biol. 1493:1.
  3. Schultze, W. et al. (2001) J. Neurochem. 78:482.
  4. Burkhardt, C. et al. (2005) FEBS Lett. 579:3821.
  5. Ben-Gigi, L. et al. (2015) eNeuro. 2:1.
  6. Nakagawa, Y. et al. (2011) J. Immunol. 186:2881.
  7. Nagai, H. et al. (2007) Oncogene. 26:4025.

Alternate Names

SEMA4B, SEMAC, SemC

Entrez Gene IDs

10509 (Human); 20352 (Mouse); 293042 (Rat)

Gene Symbol

SEMA4B

UniProt

Product Documents for Recombinant Human Semaphorin 4B Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Semaphorin 4B Fc Chimera Protein, CF

For research use only

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