Recombinant Human SCF/c-kit Ligand Biotinylated Protein, CF

Name: Recombinant Human SCF/c-kit Ligand Biotinylated Protein, CF
Brand: R&D Systems
SKU: BT11010

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BT11010

Product Datasheet / COA / SDS

Biotinylated

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Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	P21583.1
Structure / Form:	Biotinylated via amines
Applications:	Bioactivity

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Product Specifications for Recombinant Human SCF/c-kit Ligand Biotinylated Protein, CF

Source	Human embryonic kidney cell, HEK293-derived human SCF/c-kit Ligand protein Glu26-Ala189
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu26
Predicted Molecular Mass	19 kDa
SDS-PAGE	20-35 kDa, under reducing conditions.
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human CD117/c-kit Fc Chimera (Catalog # 10961-SR) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human SCF/c-kit Ligand binds with an ED₅₀ of 1.00-6.00 ng/mL.

Scientific Data Examples for Recombinant Human SCF/c-kit Ligand Biotinylated Protein, CF

	Biotinylated Recombinant Human SCF/c‑kit Ligand Protein Binding Activity. When Recombinant Human CD117/c-kit Fc Chimera (10961-SR) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human SCF/c-kit Ligand Protein binds with an ED₅₀ of 1.00‑6.00 ng/mL.
	Biotinylated Recombinant Human SCF/c‑kit Ligand Protein SDS-PAGE. 2 μg/lane of Biotinylated Recombinant Human SCF/c‑kit Ligand Protein (Catalog # BT11010) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 20-35 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT11010

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: SCF/c-kit Ligand

Stem cell factor (SCF) is a potent hematopoietic growth factor required in regulating both embryonic and adult hematopoiesis. SCF protein promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). And SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (9). Stem cell factor is an important cytokine for ex vivo clinical applications. Along with other cytokines, SCF is used in the culture and expansion of hematopoietic stem cells (HSCs) as well as for proliferation and differentiation of both myeloid and erythroid progenitor cells. Mature stem cell factor consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane domain, and a 36 aa cytoplasmic tail (10). The ECD shows both N linked and O-linked glycosylation (11). SCF protein exists in two forms, a membrane-bound form and a proteolytically processed soluble form that lacks the transmembrane domain and cytoplasmic tail. The soluble form is created by proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releasing a 25 kDa soluble SCF protein which is comparable to the only form produced by Steel-dickie mutant mice (12, 13). There is also an alternately spliced isoform of human SCF that lacks 28 amino acids that encompasses the primary proteolytic recognition site (14). This form cannot be cleaved and is only membrane bound. SCF binds to C-kit (CD117). C-kit is expressed on many different cell types including HSCs, mast cells, germ cells, and melanocytes. Binding of SCF to C-kit induces receptor dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain (15). Tyrosine phosphorylation initiates multiple signaling pathways including RAS, PI3 kinase, Src, and JAK/STAT. Stem cell factor is highly conserved among mammals. Human SCF protein shares 79% 87% aa sequence identity with dog, cat, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (10).

Item	Value
References	Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. Kapur, R. et al. (2002) Blood 100:1287. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117. Martin, F.H. et al. (1990) Cell 63:203. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671. Anderson, D.M. et al. (1991) Cell Growth Differ. 2:373. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
Long Name	Stem Cell Factor
Entrez Gene IDs	4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)
Alternate Names	DCUA, DFNA69, DKFZp686F2250, FPH2, FPHH, KIT ligand, KITLG, KL-1, Kitl, MGF, MGFSHEP7, Mast cell growth factor, SCF, SCFStem cell factor, SFc-Kit ligand, SHEP7, SLF, c-kit Ligand, familial progressive hyperpigmentation 2, steel factor

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