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Recombinant Human CD117/c-kit Fc Chimera Protein, CF

Catalog # 10961-SR | R&D Systems, Inc. a Bio-Techne Brand
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10961-SR-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD117/c-kit protein
Human SCFR
(Gln26-Thr520)
Accession # P10721.1
IEGRMD Human IgG1 Fc
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln26

Predicted Molecular Mass

82 kDa

SDS-PAGE

100-120 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit SCF-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 30.0‑180 ng/mL.

Scientific Data Images for Recombinant Human CD117/c-kit Fc Chimera Protein, CF

Recombinant Human CD117/c-kit Fc Chimera Protein Bioactivity.

Recombinant Human CD117/c-kit Fc Chimera Protein (Catalog # 10961-SR) inhibits SCF-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 30.0-180 ng/mL.

Recombinant Human CD117/c-kit Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human CD117/c-kit Fc Chimera Protein (Catalog # 10961-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-120 kDa and 200-240 kDa, respectively.

Formulation, Preparation and Storage

10961-SR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem cell factor receptor (SCFR), also known as c-Kit and CD117, is a type III receptor tyrosine kinase that acts as the receptor for the cytokine SCF. Human SCFR is expressed in embryonic stem cells and is involved in regulation of cell survival and proliferation, hematopoiesis, gametogenesis, and melanogenesis (1-3). Mature human SCFR consists of an extracellular domain (ECD) with five tandem immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic region with a split tyrosine kinase domain. Within the ECD, human SCFR shares 73% and 76% amino acid sequence identity with mouse and rat SCFR, respectively. Several SCFR isoforms, produced by alternative mRNA splicing, have been identified in humans and can be characterized by the presence or absence of a tetrapeptide sequence (GNNK) in the juxtamembrane region of the ECD (4, 5). Binding of SCFR to SCF triggers receptor dimerization and activates downstream signaling (6). SCF is a primary growth and activation factor for mast cells and eosinophils and SCFR expression on mast cells enables them to infiltrate SCF‑secreting tumors where they promote tumor growth and induce local immune suppression (7, 8). SCFR is up regulated on dendritic cells by Th2- or Th17-biasing stimuli, and it is required for subsequent dendritic cell induction of Th2 and Th17 responses (9). SCFR protects vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction (10, 11). Mutations or deletions of SCFR cause a wide variety of malignancies as well as pigmentation disorders and sterility (12).

References

  1. Roskoski, R. et al. (2005) Bioche. & Biophy Res Comm. 338:1307.
  2. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
  3. Kimura, Y. et al. (2011) PLoS ONE 6:e26918.
  4. Lennartsson, J. and Rönnstrand L. (2012) Physiol Rev. 92:1619.
  5. Caruana, G. et al. (1999) Oncogene 18:5573.
  6. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
  7. Jamur, M.C. and C. Oliver (2011) Front. Biosci. (School Ed.) 3:1390.
  8. Huang, B. et al. (2008) Blood 112:1269.
  9. Krishnamoorthy, N. et al. (2008) Nat. Med. 14:565.
  10. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  11. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
  12. Sartini, S. et al. (2011) Curr. Med. Chem. 18:2893.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Gene Symbol

KIT

UniProt

Product Documents for Recombinant Human CD117/c-kit Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD117/c-kit Fc Chimera Protein, CF

For research use only

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