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Recombinant Human Ret His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10302-CR

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10302-CR-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Ret protein
Leu29-Arg635, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu29

Predicted Molecular Mass

69 kDa

SDS-PAGE

101-113 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Ret His-tag (Catalog # 10302-CR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human GDNF (Catalog # 212-GD) binds with an ED50 of 0.03-0.3 μg/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714-GR).

Scientific Data Images for Recombinant Human Ret His-tag Protein, CF

Recombinant Human Ret His-tag Protein Binding Activity

Recombinant Human Ret His-tag Protein Binding Activity

When Recombinant Human Ret His-tag (Catalog # 10302-CR) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human GDNF (Catalog # 212-GD) binds with an ED50 of 0.03-0.3 µg/mL in the presence of Recombinant Human GFR alpha-1 Fc Chimera (Catalog # 714-GR).
Recombinant Human Ret His-tag Protein SDS-PAGE

Recombinant Human Ret His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Ret His-tag (Catalog # 10302-CR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 101-113 kDa.

Formulation, Preparation and Storage

10302-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ret

Receptor tyrosine-protein kinase, also known as Ret, is encoded by the C-RET proto-oncogene. Mutations of the C-RET gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung's disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma (1, 2, 3). Human C-RET cDNA encodes a 1115 amino acid (aa) with a 28 aa signal peptide, a 679 aa cysteine-rich extracellular domain that contains an membrane anchored Ret-Cadherin 120 Da fragment, and a 456 aa rcytoplasmic domain with a soluble Ret kinase fragment (4). Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (5).

References

  1. Ma, Q. (2009) Neuron 64:773.
  2. Carlomagno, F. et al. (1998) Endocrinology 139:3613.
  3. Cabrera, J.R. et al. (2011) J. Biol. Chem. 286:14628.
  4. Iwamoto, T. et al. (1993) Oncogene 8:1087.
  5. Yang, L. et al. (2017) Nat. Med. 23:1158.

Long Name

Proto-oncogene Tyrosine-protein Kinase Receptor Ret

Alternate Names

CDHF12MTC1, CDHR16, EC 2.7.10, Hirschsprung disease 1, Hirschsprung disease), MEN2A, MEN2B, multiple endocrine neoplasia and medullary thyroid carcinoma 1, ret proto-oncogene, RET transforming sequence

Entrez Gene IDs

5979 (Human); 19713 (Mouse)

Gene Symbol

RET

UniProt

Additional Ret Products

Product Documents for Recombinant Human Ret His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Ret His-tag Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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