Recombinant Human Protein S/PROS1, CF

Name: Recombinant Human Protein S/PROS1, CF
Brand: R&D Systems
SKU: 9489-PS
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9489-PS

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Key Product Details

Source:	NS0
Accession #:	P07225
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Protein S/PROS1, CF

Product Specifications for Recombinant Human Protein S/PROS1, CF

Source	Mouse myeloma cell line, NS0-derived human Protein S/PROS1 protein Ala42-Trp670, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala42
Predicted Molecular Mass	71 kDa
SDS-PAGE	66-93 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human Tyro3/Dtk Fc Chimera (Catalog # 859-DK) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Protein S/PROS1 binds with an ED₅₀ of 0.2-1.2 μg/mL.

Scientific Data Examples for Recombinant Human Protein S/PROS1, CF

	Recombinant Human Protein S/PROS1 Bioactivity When Recombinant Human Tyro3/Dtk Fc Chimera (Catalog # 859-DK) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human Protein S/PROS1 (Catalog # 9489-PS) binds with an ED₅₀ of 0.2-1.2 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9489-PS

Formulation	Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl₂.
Reconstitution	Reconstitute at 1 mg/mL in water. Reconstitution Buffer Available: Size / Price Qty Loading... RB07:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protein S/PROS1

Anticoagulant Protein S (PROS1) is a 71 kDa plasma vitamin K-dependent glycoprotein characterized by the presence of the post-translational modification of specific glutamic acid residues to gamma-carboxyglutamic acid (Gla) in the N-terminal region. In addition to the N-terminal Gla domain, mature PROS1 contains a thrombin‑sensitive thumb loop, four tandem EGF-like domains and a C-terminal sex hormone-binding globulin (SHBG) domain composed of two Laminin G (LG) domains (1). Human PROS1 shares 80% amino acid sequence identity with mouse PROS1. Hundreds of mutations have been reported throughout all the domains of the protein, typically resulting in PROS1 deficiency and an increase in the risk of thrombophilia (2, 3). PROS1 is expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response (4-9). PROS1 exists in plasma both in complex with C4b-binding protein (C4BP) (60%) and in a free form (40%). Both forms of PROS1 have anti-coagulant activity either directly through inhibition of Factor X systems and prothrombinase while in complex with C4BP (5, 10) or in its well-established role as a cofactor of activated protein C (APC) inactivation of procoagulants FVa and FVIIIa (4). The free form of PROS1 is also a ligand for a subfamily of receptor tyrosine kinases known as TAMs, which is composed of TYRO3, AXL, and MERTK (11). PROS1 binds these tyrosine kinase receptors through its LG domains to activate downstream signaling pathways involved in tumor development and progression (7, 8, 11, 12).

Item	Value
References	Dahlback, B. et al. (1986) Proc. Natl. Acad. Sci. 83:4199. Garcia de Frutos, P. et al. (2007) J. Thromb. Haemost. 98:543. Heeb, M. J. (2008) Expert Rev. Hematol. 1:9. Walker, F.J. (1984 Sem. Thromb. Hemosta. 10:131. Heeb, M. J. et al. (2004) J. Thromb. Haemost. 2:1766. Che Mat, M. F. (2016) Int. J. Oncol. 49:2359. Abboud-Jarrous, G. et al. (2017) Oncotarget. 8:13986. Qin, J. et al. (2016) Sci. Rep. 6:26662. Davra, V. et al. (2016) Cancers (Basel). 8:E107. Hackeng, T. M. et al. (1994) J. Biol. Chem. 269:21051. Tsou, W.I. et al. (2014) J. Biol. Chem. 289:25750. Linger, R.M.A. et al. (2008) Adv. Cancer Res. 100:35.
Entrez Gene IDs	5627 (Human); 19128 (Mouse); 81750 (Rat)
Alternate Names	EC 3.4.21, PROS, PROS1, PS21, PS22, PS23, PS24, PS25, PSA, Protein S, protein S (alpha), protein Sa, vitamin K-dependent plasma protein S, vitamin K-dependent protein S

Citations for Recombinant Human Protein S/PROS1, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Protein S/PROS1, CF include:

Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

H Wu et al. (2021), Mer regulates microglial/macrophage M1/M2 polarization and alleviates neuroinflammation following traumatic brain injury Journal of Neuroinflammation, 18(1):2.
PMID: 33402181

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Name: Leiming Li

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 10/11/2018

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Product Documents for Recombinant Human Protein S/PROS1, CF

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