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Recombinant Human Podocalyxin Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1658-PD

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1658-PD-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Podocalyxin protein
Ser23-Arg427 and a C-terminal Asp and 10-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser23

Predicted Molecular Mass

43 kDa (monomer)

SDS-PAGE

125-165 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin.
The ED50 for this effect is 0.08-0.4 μg/mL.

Scientific Data Images for Recombinant Human Podocalyxin Protein, CF

Podocalyxin Inhibits Cell Adhesion to P-Selectin.

Podocalyxin Inhibits Cell Adhesion to P-Selectin.

Immobilized Recombinant Human Podocalyxin inhibits the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin.

Formulation, Preparation and Storage

1658-PD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin (PODXL), also known as Podocalyxin-like protein 1 (PCLP-1), is a heavily glycosylated transmembrane sialoprotein in the CD34 and Endoglycan family (1). Mature human Podocalyxin consists of a 406 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 75 aa cytoplasmic domain (2). Within the ECD, human Podocalyxin shares 31% aa sequence identity with mouse and rat Podocalyxin. Alternative splicing of human Podocalyxin generates an additional isoform with a 33 aa deletion in the ECD. Podocalyxin is expressed on glomerular and vascular endothelial cells (2, 3), neurons (4), hematopoietic, mesenchymal, and cardiac stem cells (5-8), and metastatic carcinoma and glioblastoma tumor cells (9-11). A soluble form of Podocalyxin can be released into the urine of women with pre-eclampsia (12). Podocalyxin promotes platelet activation (13), neurite outgrowth, branching, and synaptogenesis (4), homing of hematopoietic progenitor cells to the bone marrow and spleen (6), and engraftment of mesenchymal and cardiac stem cells into ischemic heart and kidney (7, 8). Its interactions with L-Selectin and E-Selectin mediate the tethering of lymphocytes and metastatic tumor cells to the vascular endothelium (3, 9, 10). Podocalyxin can be expressed as a 160-165 kDa molecule or as even larger glycoforms (2, 3, 10, 14). In humans, an approximately 200 kDa glycoform contains the carbohydrate antigen recognized by TRA-1-60 and TRA-1-81 antibodies used for the identification of undifferentiated pluripotent embryonic stem cells (14).

References

  1. Nielsen, J.S. and K.M. McNagny (2009) J. Am. Soc. Nephrol. 20:1669.
  2. Kershaw, D.B. et al. (1997) J. Biol. Chem. 272:15708.
  3. Sassetti, C. et al. (1998) J. Exp. Med. 187:1965.
  4. Vitureira, N. et al. (2010) PLoS ONE 8:e12003.
  5. Zhang, H. et al. (2014) Stem Cells 32:191.
  6. Doyonnas, R. et al. (2005) Blood 105:4170.
  7. Lee, R-H. et al. (2009) Blood 113:816.
  8. Moscoso, I. et al. (2013) J. Tissue Eng. Regen. Med. Epub. PMID 23897803.
  9. Dallas, M.R. et al. (2012) Am. J. Physiol. Cell Physiol. 303:C616.
  10. Thomas, S.N. et al. (2009) Am. J. Cell Physiol. 296:C505.
  11. Binder, Z.A. et al. (2013) PLoS ONE 8:e75945.
  12. Wang, Y. et al. (2012) Am. J. Physiol. Renal Physiol. 302:F1084.
  13. Alonso-Martin, S. et al. (2010) Thromb. Res. 125:e300.
  14. Schopperle, W.M. and W.C. DeWolf (2007) Stem Cells 25:723.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Entrez Gene IDs

5420 (Human); 27205 (Mouse)

Gene Symbol

PODXL

UniProt

Additional Podocalyxin Products

Product Documents for Recombinant Human Podocalyxin Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Podocalyxin Protein, CF

For research use only

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