Chinese Hamster Ovary cell line, CHO-derived human Plexin A3 protein Asn20-Ala1220, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
135-160 kDa, under reducing conditions
Measured by its ability to bind Recombinant Human Semaphorin 3F Fc Chimera (Catalog # 9878-S3).
Recombinant Human Plexin A3 His-tag Protein, CF Scientific Data Examples
Recombinant Human Plexin A3 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human Plexin A3 His-tag Protein (10519-PA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 135-160 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin A3
Plexin A3, also known as Plexin-4, is a member of the large Plexin family of conserved transmembrane receptors that mediate Semaphorin functions (1, 2). There are four classes plexins (A, B, C, and D) and they share a similar domain structure: An extracellular domain (ECD) that binds the semaphorin ligand, a single transmembrane domain and an approximately 600 residue-long cytoplasmic region (2). The ECD of mature, human plexinA-3 consists of a Sema domain and four IPT (Ig-like, plexins, transcription factors) domains and shares 93% amino acid identity with both mouse and rat PlexinA-3. Plexin A3 is a coreceptor for class 3 semaphorins and plays an important role in axon guidance in the developing nervous system and regulates the migration of sympathetic neurons (3, 4). Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin (4). Plexin A3 is required for normal dendrite spine morphology in pyramidal neurons and may play a role in regulating semaphorin-mediated programmed cell death in the developing nervous system (5). The cytoplasmic domain of plexinA-3 is required for the activation of down-stream signaling events in the cytoplasm (6).
Pascoe, H.G. et al. (2015) Prog Biophys Mol Biol. 118(3):161.
Tamagnone L et al. (1999) Cell. 99:71.
Alto, L. T. et al. (2017) Methods Mol Biol. 1493:1.
Yaron, A. et al. (2005) Neuron. 45:513.
Cheng, H. J. et al. (2001) Neuron. 32:249.
He H. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:15610.
Entrez Gene IDs
55558 (Human); 18846 (Mouse); 309280 (Rat)
HSSEXGENE, PLXN4, PLXNA3, Plexin A3, Plxn3, SEX, SEXPLEXIN-A3,6.3, Semaphorin receptor SEX, Sex chromosome X transmembrane protein of HGF receptor family 3, XAP-6, plexin-4, plexin-A3
Citations for Recombinant Human Plexin A3 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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